Forced Expression of Foxg1 in the Cortical Hem Leads to the Transformation of Cajal-Retzius Cells into Dentate Granule Neurons.
Forced Expression of Foxg1 in the Cortical Hem Leads to the Transformation of Cajal-Retzius Cells into Dentate Granule Neurons.
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Foxg1 在皮质下缘的强制表达导致 Cajal-Retzius 细胞转化为齿状颗粒神经元。
DOI：
10.3390/jdb6030016
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发表时间：
2018-06-26
影响因子：
2.7
通讯作者：
Zhao C
中科院分区：
文献类型：
--
作者：
Liu B;Xiao H;Zhao C
富含Wnt和bmp的皮质边缘已被证明对端脑的模式形成至关重要，对海马的诱导尤为重要。同时，皮质下缘是Cajal-Retzius细胞的来源之一。许多Cajal-Retzius细胞产生于边缘，并分布于端脑的中端-尾侧表面。然而，其维持机制尚不清楚。在这项研究中，我们建立了一个转基因小鼠系CAG-loxp-stop-loxp-Foxg1-IRES-EGFP。通过将Fzd10CreERTM与该细胞系杂交，并结合他莫昔芬诱导，Foxg1从胚胎10.5天（E10.5）起在下缘异位表达。我们发现hema来源的Cajal-Retzius细胞转化为齿状颗粒神经元，并伴有Lhx2的异位表达。而下摆形态无明显变化。下摆特异性标记Wnt3a和Wnt2b略有下调。我们的结果表明Foxg1足以诱导Lhx2在下颌骨背侧的表达。Lhx2的异位和Wnt信号的减少都可能导致细胞命运的转变。我们的研究为下摆维持的机制提供了新的见解。
The Wnt- and BMP-rich cortical hem has been demonstrated to be critical for the pattern formation of the telencephalon, and it is particularly important for the induction of the hippocampus. Meanwhile, the cortical hem is one of the sources of Cajal-Retzius cells. Many Cajal-Retzius cells are produced in the hem and populated to the media-caudal surface of the telencephalon. However, the mechanism of the maintenance of the hem remain unclear. In this study, we generated a transgenic mouse line CAG-loxp-stop-loxp-Foxg1-IRES-EGFP. By crossing Fzd10CreERTM with this line, combined with tamoxifen induction, Foxg1 was ectopically expressed in the hem from embryonic day 10.5 (E10.5) onwards. We have found the hem-derived Cajal-Retzius cells were transformed into dentate granule neurons accompanied with ectopic expression of Lhx2. However, the morphology of the hem displayed no obvious changes. The hem specific markers, Wnt3a and Wnt2b, were slightly downregulated. Our results indicate that Foxg1 is sufficient to induce the expression of Lhx2 in the dorsal part of the hem. The ectopic Lhx2 and decreased Wnt signals may both contribute to the cell fate switch. Our study provides new insight into the mechanism underlying the maintenance of the hem.