Neurocan in the embryonic avian heart and vasculature.
Neurocan in the embryonic avian heart and vasculature.
复制标题
胚胎禽心脏和脉管系统中的 Neurocan。
DOI：
10.1002/ar.a.10067
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发表时间：
2003
期刊：
影响因子：
--
通讯作者：
Hoffman,Stanley
中科院分区：
文献类型：
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作者：
Mishima,Noboru;Hoffman,Stanley
硫酸软骨素蛋白聚糖（CSPG）神经聚糖以前被认为是神经系统特异性的。然而，我们在胚胎心脏和脉管系统中发现了神经能。在11期鹌鹑胚胎中，neurocan在心肌、背心系膜、心脏形成野、内脏中胚层和胚胎外空泡附近显著表达，在心内膜中表达水平较低。神经can染色与血管内皮细胞QH1染色的比较表明，神经can经常由内皮细胞邻近的细胞表达，而不是内皮细胞本身表达。在某些情况下，分散的细胞亚群在细胞场中呈神经can阳性，否则在形态学上表现一致。在发育后期，神经能蛋白的表达相对局限于神经系统。然而，即使在10天的胚胎中，神经can也在组织中的绒毛膜-尿囊膜中表达，该组织将紧密排列的小直径血管分开。总之，我们的研究结果表明，neurocan可能在发育过程中作为调节血管模式的屏障发挥作用。环境科学学报，2003,27(2):556-562。©2003 Wiley‐Liss, Inc。
The chondroitin sulfate proteoglycan (CSPG) neurocan was previously considered to be nervous‐system specific. However, we have found neurocan in the embryonic heart and vasculature. In stage 11 quail embryos, neurocan was prominently expressed in the myocardium, dorsal mesocardium, heart‐forming fields, splanchnic mesoderm, and vicinity of the extraembryonic vaculature, and at lower levels in the endocardium. A comparison of neurocan staining with QH1 staining of vascular endothelial cells demonstrates that neurocan is frequently expressed by cells adjacent to endothelial cells, rather than by endothelial cells themselves. In some cases, a dispersed subset of cells are neurocan‐positive in a field of cells that otherwise appear uniform in morphology. Later in development, neurocan expression becomes relatively limited to the nervous system. However, even in 10‐day embryos, neurocan is expressed in the chorio‐allantoic membrane in the tissue that separates closely packed, small‐diameter blood vessels. In summary, our results suggest that neurocan may function as a barrier that regulates vascular patterning during development. Anat Rec Part A 272A: 556–562, 2003. © 2003 Wiley‐Liss, Inc.