KAR5 encodes a novel pheromone-inducible protein required for homotypic nuclear fusion
KAR5 encodes a novel pheromone-inducible protein required for homotypic nuclear fusion
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DOI：
10.1083/jcb.139.5.1063
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发表时间：
1997-12-01
影响因子：
7.8
通讯作者：
Rose, MD
中科院分区：
文献类型：
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作者：
Beh, CT;Brizzio, V;Rose, MD
KAR5 是核配体（酵母交配过程中的核融合过程）过程中膜融合所必需的。为了研究核融合的分子机制，我们克隆并表征了KAR5基因及其产物。 KAR5 是一个非必需基因，缺失突变会导致酵母细胞核同型融合的双侧缺陷。 KARS 编码一种新蛋白，与粟酒裂殖酵母中的一种蛋白相似，后者可能在核融合中发挥类似的作用。 Kar5p 作为信息素响应途径的一部分被诱导，表明该蛋白在核膜融合交配过程中发挥着独特的特定作用。 Kar5p 是一种膜蛋白，其可溶性结构域完全包含在内质网的管腔内。 In pheromonetreated cells, Kar5p was localized to the vicinity of the spindle pole body, the initial site of fusion between haploid nuclei during karyogamy.我们认为 Kar5p 是完成核膜融合所必需的，并且可能在膜融合复合物的组织中发挥作用。
KAR5 is required for membrane fusion during karyogamy, the process of nuclear fusion during yeast mating. To investigate the molecular mechanism of nuclear fusion, we cloned and characterized the KAR5 gene and its product. KAR5 is a nonessential gene, and deletion mutations produce a bilateral defect in the homotypic fusion of yeast nuclei. KARS encodes a novel protein that shares similarity with a protein in Schizosaccharomyces pombe that may play a similar role in nuclear fusion. Kar5p is induced as part of the pheromone response pathway, suggesting that this protein uniquely plays a specific role during mating in nuclear membrane fusion. Kar5p is a membrane protein with its soluble domain entirely contained within the lumen of the endoplasmic reticulum. In pheromonetreated cells, Kar5p was localized to the vicinity of the spindle pole body, the initial site of fusion between haploid nuclei during karyogamy. We propose that Kar5p is required for the completion of nuclear membrane fusion and may play a role in the organization of the membrane fusion complex.