Single molecule analysis of RNA polymerase elongation reveals uniform kinetic behavior
Single molecule analysis of RNA polymerase elongation reveals uniform kinetic behavior
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DOI：
10.1073/pnas.212358999
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发表时间：
2002-10-15
影响因子：
11.1
通讯作者：
Wang, MD
中科院分区：
文献类型：
--
作者：
Adelman, K;La Porta, A;Wang, MD
通过使用单分子测量，我们证明了单个大肠杆菌RNA聚合酶分子的延伸动力学是非常均匀的。我们发现没有证据表明在RNA聚合酶之间有明显的延伸状态。相反，观察到的转录率异质性是由暂停频率和持续时间的统计差异引起的。当转录一个没有强停顿位点的基因时，RNA聚合酶分子表现出在时间和距离上随机分布的短暂停顿。主动延伸模式和暂停状态之间的转换在我们测量的分辨率内是瞬时的(
By using single-molecule measurements, we demonstrate that the elongation kinetics of individual Escherichia coli RNA polymerase molecules are remarkably homogeneous. We find no evidence of distinct elongation states among RNA polymerases. Instead, the observed heterogeneity in transcription rates results from statistical variation in the frequency and duration of pausing. When transcribing a gene without strong pause sites, RNA polymerase molecules display transient pauses that are distributed randomly in both time and distance. Transitions between the active elongation mode and the paused state are instantaneous within the resolution of our measurements (