Pandinus imperator scorpion venom blocks voltage-gated K+ channels in human lymphocytes.
Pandinus imperator scorpion venom blocks voltage-gated K+ channels in human lymphocytes.
复制标题
Pandinus imperator 蝎毒可阻断人类淋巴细胞中的电压门控 K 通道。
DOI：
10.1006/bbrc.1997.8018
复制
发表时间：
1998
期刊：
影响因子：
--
通讯作者：
GasparJr,R
中科院分区：
文献类型：
--
作者：
PeterJr,M;Varga,Z;Panyi,G;Bene,L;Damjanovich,S;Pieri,C;Possani,LD;GasparJr,R
采用膜片钳技术，测定了大熊猫蝎毒对人外周血淋巴细胞全细胞n型钾电流的阻断作用，其Kd值为0.02 μg/ml，呈剂量依赖性。K+通道阻滞是瞬时的，并且可以通过用无毒液的细胞外溶液洗涤来去除。毒液引起的阻滞与膜电位无关。毒液不影响K+通道的激活和失活动力学，但是，加速从失活恢复。从P.在人淋巴细胞中，欧前胡毒可有效阻断Kv1.3通道，Kd值分别为9.7 nM、50 pM和0.5 nM。流式细胞仪膜电位测定表明，Pi 2，最有效的肽毒素的P。欧前胡毒，根据其K+通道阻断作用使人淋巴细胞去极化。
Using the patch-clamp technique, we determined thatPandinus imperatorscorpion venom blocked whole-cell n-type K+currents in human peripheral blood lymphocytes in a dose-dependent manner with Kd= 0.02 μg/ml. K+channel block was instantaneous and removable by washing with venom-free extracellular solution. The venom-induced block was independent of membrane potential. The venom did not influence activation and inactivation kinetics of the K+channels, however, accelerated recovery from inactivation. Purified peptides Pi1, Pi2, and Pi3 from theP. imperatorvenom powerfully blocked Kv1.3 channels in human lymphocytes with Kdvalues of 9.7 nM, 50 pM, and 0.5 nM, respectively. Flow cytometric membrane potential measurements with the oxonol dye showed that Pi2, the most effective peptide toxin of theP. imperatorvenom, depolarizes human lymphocytes in accordance with its K+channel blocking effect.