Biochemical Analyses of Trichogramma dendrolimi (Hymenoptera: Trichogrammatidae) in Vitro and in Vivo Rearing for 10 Generations
Biochemical Analyses of Trichogramma dendrolimi (Hymenoptera: Trichogrammatidae) in Vitro and in Vivo Rearing for 10 Generations
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DOI：
10.1653/024.098.0315
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发表时间：
2015-09
期刊：
影响因子：
--
通讯作者：
Xin Lü;Shichou Han;Liying Li
中科院分区：
文献类型：
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作者：
Xin Lü;Shichou Han;Liying Li
松毛虫赤眼蜂Trichogramma dendrolimi Matsumura是一种重要的经济卵寄生蜂，寄主范围广，可大量生产用于生物防治。随着离体饲养技术的发展，已经开发出一种人工培养基，被认为具有大规模生产寄生蜂的潜力。我们试图通过生物化学分析来评估人工饲养介质的有效性，而不仅仅是通过比较饲养黄蜂的生物学参数。特别地，进行本研究以确定用于大量饲养T的人工培养基和天然宿主之间的营养物比例的差异。Dendrolimi的尸体的生化分析。测定了T.比较了在优化的人工培养基中连续饲养的松毛虫与在柞蚕卵上饲养的松毛虫。与体内培养的成虫相比，体外培养的成虫蛋白质含量较低，脂肪和海藻糖含量较高，海藻糖酶活性较高。在人工培养基中饲养的成虫与在自然寄主卵中饲养的成虫在10代以上的比较中发现了不同的甚至相反的生化参数的进化。结果表明，人工培养液中存在一个非最佳组成。Dendrolimi，并可以通过改变培养基的组成，特别是其蛋白质浓度和脂质含量来改善。生物化学分析可作为其它食虫昆虫人工饲养基质优化的一种有效方法。
Abstract Trichogramma dendrolimi Matsumura (Hymenoptera: Trichogrammatidae) is an economically important egg parasitoid with a wide host range and is mass produced for biological control programs. With the development of in vitro rearing techniques, an artificial medium has been developed that is believed to hold potential for the large-scale production of the parasitoid. We sought to evaluate the effectiveness of an artificial rearing medium by including biochemical analyses and not just by comparing the biological parameters of reared wasps. In particular, this study was conducted to determine differences in proportions of nutrients between the artificial medium and a natural host used for mass rearing T. dendrolimi by biochemical analyses of carcasses of the latter. Biochemical parameters including protein concentration, lipid content, trehalose content, and trehalase activity in T. dendrolimi continuously reared in an optimized artificial medium versus on Antheraea pernyi Guérin-Méneville (Lepidoptera: Saturniidae) eggs were compared. The adults produced in vitro showed lower protein concentrations and higher lipid and trehalose contents and trehalase activity compared with those reared in vivo. Different—and even contrary—evolution of biochemical parameters was found when comparing adults reared in the artificial medium with those reared in natural host eggs over 10 generations. The results suggested that the artificial medium had a non-optimal composition for the in vitro rearing of T. dendrolimi, and that it could be improved by altering the composition of the medium, especially its protein concentration and lipid content. Biochemical analysis may be a useful method for optimizing artificial rearing media for other entomophagous insect species.