A simple method of chromosome doubling using colchicine in Torenia (Linderniaceae), and the behavior of meiotic chromosomes in amphidiploids
A simple method of chromosome doubling using colchicine in Torenia (Linderniaceae), and the behavior of meiotic chromosomes in amphidiploids
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使用秋水仙碱对蓝猪草（Linderniaceae）进行染色体加倍的简单方法，以及双二倍体中减数分裂染色体的行为
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发表时间：
2011
期刊：
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通讯作者：
H. Tsujimoto
中科院分区：
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作者：
Jiraporn Jiranapapan;S. Kikuchi;B. Manochai;T. Taychasinpitak;Hiroyuki Tanaka;H. Tsujimoto
虽然它们的F_1代分别含有2n=18和2n=16的染色体，但由于种间配对，它们的F_1代有8条二价体染色体和1条单价体染色体。为了阐明染色体在双二倍体中的行为，我们开发了一种简单的染色体加倍方法，即在离体叶中应用秋水仙素结合不定芽诱导的方法。15μ摩尔秋水仙碱处理2天，多倍体植株出现率最高(8%)。双二倍体的小细胞表现出一定程度的配对(即二价和多价染色体的出现)，包括种内配对和种间配对。这一结果支持这样的假设，即塔尔尼尔虫和贝氏锥虫有8条同源染色体，而塔尔尼耶尔锥虫有一条未知来源的额外染色体。
Although Torenia fournieri (Linderniaceae) and Torenia baillonii carry 2n=18 and 2n=16 chromosomes, respectively, their F1 hybrid showed eight bivalent chromosomes with chiasmata as a result of interspecific pairing and one univalent chromosome from T. fournieri. To elucidate the behavior of the chromosomes in the amphidiploids, we developed a simple method of chromosome doubling by the application of colchicine combined with the induction of adventitious buds in detached leaves. The frequency of polyploid plants was highest (8%) in the treatment with 15 μmol mol colchicine for 2 days. The meiocytes of the amphidiploids showed extent pairing (i.e., the appearance of bivalent and multivalent chromosomes), including intraspecific and interspecific pairings. This result supports the hypothesis that T. fournieri and T. baillonii have eight homologous chromosomes and that T. fournieri possesses an extra chromosome of unknown origin.