Enhanced gene transfection efficiency in CD13-positive vascular endothelial cells with targeted poly(lactic acid)-poly(ethylene glycol) nanoparticles through caveolae-mediated endocytosis
Enhanced gene transfection efficiency in CD13-positive vascular endothelial cells with targeted poly(lactic acid)-poly(ethylene glycol) nanoparticles through caveolae-mediated endocytosis
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DOI：
10.1016/j.jconrel.2011.02.027
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发表时间：
2011-04-30
影响因子：
10.8
通讯作者：
Zhang, Na
中科院分区：
文献类型：
--
作者：
Liu, Chunxi;Yu, Wangyang;Zhang, Na
如何有效地将治疗基因导入目标细胞是非病毒载体广泛应用的关键挑战。在纳米颗粒表面引入配体引导基因载体靶向小泡介导的内吞作用可能是一种有效的基因转染策略。最近，为了提高小囊泡介导的内吞作用的可能性，我们制造了一个肽靶向基因载体，用于高效的受体介导的细胞内递送。利用环Asn-Gly-Arg （cNGR）肽将负载基因的聚乳酸-聚乙二醇纳米粒子（PLA-PEG NPs）靶向到过表达CD13的HUVEC上。以6-月桂氧己基裂解酸酯（LHLN）为阳离子表面活性剂，cNGR修饰的PLA-PEG NPs （cNGR- peg - pla NPs）能够将DNA络合并压实成均匀的小尺寸配合物(
The efficient delivery of therapeutic gene into cells of interest is a critical challenge to broad application of non-viral vectors. The approach of introducing ligands that lead gene vectors to target caveolae-mediated endocytosis on nanoparticle surface might serve as a promising strategy for the effective gene transfection. Recently, in an attempt to enhance the possibility of caveolae-mediated endocytosis, we fabricated a peptide-targeted gene vector for highly efficient receptor-mediated intracellular delivery. Cyclic Asn-Gly-Arg (cNGR) peptide was used to target gene loaded poly( lactic acid)-poly(ethylene glycol) nanoparticles (PLA-PEG NPs) to HUVEC over-expressing CD13. Using 6-lauroxyhexyl lysinate (LHLN) as cationic surfactant, cNGR modified PLA-PEG NPs (cNGR-PEG-PLA NPs) were capable of complexing and compacting DNA into homogeneous small-sized complexes (