SYNTHESIS OF SUBGENOMIC MESSENGER-RNAS OF MOUSE HEPATITIS-VIRUS IS INITIATED INDEPENDENTLY - EVIDENCE FROM UV TRANSCRIPTION MAPPING
SYNTHESIS OF SUBGENOMIC MESSENGER-RNAS OF MOUSE HEPATITIS-VIRUS IS INITIATED INDEPENDENTLY - EVIDENCE FROM UV TRANSCRIPTION MAPPING
复制标题
DOI：
10.1128/jvi.39.2.401-406.1981
复制
发表时间：
1981-01-01
影响因子：
5.4
通讯作者：
VANDERZEIJST, BAM
中科院分区：
文献类型：
--
作者：
JACOBS, L;SPAAN, WJM;VANDERZEIJST, BAM
通过UV转录图谱确定了用于合成基因组大小的RNA和在小鼠肝炎病毒株A59感染的细胞中发现的6个亚基因组RNA的模板的靶大小。在感染后6 h照射感染的Sac（-）细胞，此时病毒特异性RNA合成开始呈指数增加。通过琼脂糖凝胶电泳分离后定量这些RNA，确定增加UV剂量对单个RNA合成的影响。为模板计算的UV靶标大小与RNA的物理大小几乎相同。因此，亚基因组RNA可能不是从共同的前体加工或剪接的。相反，在基因组大小的负链模板或更小的模板上独立的转录起始明显发生。
The target sizes of the templates for the synthesis of the genome-sized RNA and the 6 subgenomic RNA found in cells infected with mouse hepatitis virus strain A59 were determined by UV transcription mapping. Infected Sac(-) cells were irradiated at 6 h postinfection, the time when virus-specific RNA synthesis starts to increase exponentially. The effect of increasing UV doses on the synthesis of the individual RNA was determined by quantitation of these RNA after separation by agarose gel electrophoresis. The UV target sizes calculated for the templates were almost identical to the physical sizes of the RNA. Thus the subgenomic RNA are probably not processed or spliced from a common precursor. Rather, independent initiation of transcription on a genome-sized, negative-stranded template or on smaller templates evidently occurs.