HEPATIC CHEMILUMINESCENCE AND LIPID-PEROXIDATION IN MILD IRON OVERLOAD
HEPATIC CHEMILUMINESCENCE AND LIPID-PEROXIDATION IN MILD IRON OVERLOAD
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DOI：
10.1016/0300-483x(92)90015-7
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发表时间：
1992-11-22
期刊：
影响因子：
4.5
通讯作者：
PUNTARULO, S
中科院分区：
文献类型：
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作者：
GALLEANO, M;PUNTARULO, S
研究了铁超载对右旋糖酐铁注射后早期肝脂质过氧化和化学发光的影响。铁给药后2-6 h，肝脏总铁含量明显高于对照组。铁注入后4 ~ 6 h，光发射增加4倍。血浆GOT、GPT和LDH活性未受治疗影响，提示细胞通透性未受坏死影响。在NADPH作为辅助因子存在的情况下，铁给药后肝脏匀浆中硫代巴比妥酸反应物质（TBARS）的生成和化学发光的增加作为时间的函数。在相同的实验条件下，铁处理2 h后微粒体细胞色素P-450含量降低了40%。为了评价肝脏抗氧化防御能力，测定了过氧化氢酶、超氧化物歧化酶和谷胱甘肽过氧化物酶活性。匀浆中的谷胱甘肽过氧化物酶活性不受处理的影响。铁给药4 h后，过氧化氢酶和超氧化物歧化酶活性分别比对照组下降了25%和36%。我们的数据表明，即使肝脏保持功能，轻度铁超载后也会发生脂质过氧化。
The effect of iron-overload on both hepatic lipid peroxidation and chemiluminescence was studied in early stages after iron-dextran injection. Total hepatic iron content was markedly elevated over control values 2-6 h after iron dose. A 4-fold increase in light emission was detected after 4-6 h after iron injection. Plasma GOT, GPT and LDH activities were not affected by the treatment suggesting that cell permeability was not affected by necrosis. Increases in the generation of thiobarbituric acid reactive substances (TBARS) and chemiluminescence in liver homogenates, were determined as a function of time after iron administration, in the presence of NADPH as cofactor. Under the same experimental conditions, microsomal cytochrome P-450 content was decreased by 40%, 2 h after iron treatment. To evaluate liver antioxidant defenses, catalase, superoxide dismutase and glutathione peroxidase activities were determined. Glutathione peroxidase activity in the homogenate was not affected by the treatment. Catalase and superoxide dismutase activities declined by 25 and 36%, respectively, compared with control values 4 h after the iron dose. Our data suggest that lipid peroxidation occurs after mild iron overload even though the liver remains functional.