Production of tuna waste hydrolysates by a commercial neutral protease preparation
Production of tuna waste hydrolysates by a commercial neutral protease preparation
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DOI：
10.1016/s1381-1177(02)00203-5
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发表时间：
2002-12-02
影响因子：
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通讯作者：
Binet, A
中科院分区：
文献类型：
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作者：
Guerard, F;Guimas, L;Binet, A
鱼蛋白水解物 (FPH) 由于其作为生物活性肽来源或作为发酵培养基的含氮底物的潜在应用而受到越来越多的关注。使用商业蛋白酶“Umamizyme”水解罐头工业的废物。在 pH 7 和 45 ℃ 的 1-1 间歇式反应器中研究了酶水解。研究了工艺变量（酶/蛋白质底物比例范围为 0.1 至 1.5%（w/w）蛋白质）对蛋白水解降解程度、释放的氮和肽的分子量分布的影响。酶/底物比为1.5%，水解4小时后，水解度高达22.5%。发现水解度和氮回收率之间存在线性相关性。水解的限制因素是从在 45 摄氏度和 pH 7 下进行的一组实验中推导出来的，其中研究了中间底物和酶添加的影响。结果表明，蛋白酶“Umamizyme”在溶解金枪鱼废物方面与 Alcalase(R) 2, 4L 一样有效。然而，Umamizyme 稳定性低于 Alcalase(R) 2、4L。 (C) 2002 Elsevier Science B.V. 保留所有权利。
The fish protein hydrolysates (FPH) are of increasing interest due to their potential applications as a source of bioactive peptides or as nitrogenous substrates for the fermentation media. A waste product from the canning industry was hydrolysed using a commercial protease "Umamizyme". Enzymatic hydrolysis was investigated in a 1-1 batch reactor at pH 7 and 45 degreesC. The influence of the process variables (enzyme/protein substrate ratio ranging from 0.1 to 1.5 % (w/w) protein) was studied with regards to the extent of the proteolytic degradation, the nitrogen released and the molecular weight distribution of the peptides. A degree of hydrolysis up to 22.5% was obtained with an enzyme/substrate ratio of 1.5%, after 4 h of hydrolysis. A linear correlation was found to exist between the hydrolysis degree and the nitrogen recovery. The limiting factor of hydrolysis was deduced from a set of experiments conducted at 45 degreesC and pH 7 where the effects of intermediate substrate and enzyme addition were studied. Results showed that the protease "Umamizyme" performed as effectively as Alcalase(R) 2, 4L for the tuna waste solubilisation. However, the Umamizyme stability is lower than Alcalase(R) 2, 4L. (C) 2002 Elsevier Science B.V. All rights reserved.