Sequence and phylogenetic analysis of the S1 genome segment of turkey-origin reoviruses
Sequence and phylogenetic analysis of the S1 genome segment of turkey-origin reoviruses
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DOI：
10.1007/s11262-006-0044-1
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发表时间：
2007-10-01
期刊：
影响因子：
1.6
通讯作者：
Spackman, Erica
中科院分区：
文献类型：
--
作者：
Day, J. Michael;Pantin-Jackwood, Mary J.;Spackman, Erica
基于先前的报道，火鸡源禽呼肠孤病毒（TRV）sigma B（sigma 2）主要外衣壳蛋白基因的特征，TRV可能代表了一个新的组内的融合正呼肠孤病毒。然而，还没有来自其他TRV基因或基因组片段的序列数据被报道。由禽类呼肠孤病毒S1基因组区段编码的σ C蛋白是细胞附着蛋白，并且是禽类呼肠孤病毒的主要抗原决定簇。鸡呼肠孤病毒S1基因组片段被很好地表征，并且在来自该物种的病毒中很好地保守。本报告详细介绍了两个TRV的整个S1基因组片段的扩增、克隆和测序，以及另外五个TRV的sigma C、p10和p17基因的整个编码序列。序列分析表明，在TRV S1基因组片段编码的三种蛋白中，sigma C与鸡呼肠孤病毒参考株S1133的sigma C的氨基酸同源性最高为57%，而最相似的p10和p17蛋白与相应的S1133蛋白的同源性分别为72%和61%。最密切相关的哺乳动物呼肠孤病毒，融合性纳尔逊湾呼肠孤病毒，编码与TRV sigma C蛋白共有25%至28%氨基酸同一性的sigma C蛋白。这份报告支持了早期的建议，TRV是一个独立的病毒物种内的正呼肠孤病毒属，并可能提供一些深入了解TRV宿主特异性和发病机制。
Based on previous reports characterizing the turkey-origin avian reovirus (TRV) sigma B (sigma 2) major outer capsid protein gene, the TRVs may represent a new group within the fusogenic orthoreoviruses. However, no sequence data from other TRV genes or genome segments has been reported. The sigma C protein encoded by the avian reovirus S1 genome segment is the cell attachment protein and a major antigenic determinant for avian reovirus. The chicken reovirus S1 genome segment is well characterized and is well conserved in viruses from that species. This report details the amplification, cloning and sequencing of the entire S1 genome segment from two and the entire coding sequences of the sigma C, p10 and p17 genes from an additional five TRVs. Sequence analysis reveals that of the three proteins encoded by the TRV S1 genome segment, sigma C shares at most 57% amino acid identity with sigma C from the chicken reovirus reference strain S1133, while the most similar p10 and p17 proteins share 72% and 61% identity, respectively, with the corresponding S1133 proteins. The most closely related mammalian reovirus, the fusogenic Nelson Bay reovirus, encodes a sigma C protein that shares from 25% to 28% amino acid identity with the TRV sigma C proteins. This report supports the earlier suggestion that the TRVs are a separate virus species within the Orthoreovirus genus, and may provide some insight into TRV host specificity and pathogenesis.