Surfactant treatments alter endogenous surfactant metabolism in rabbit lungs.
Surfactant treatments alter endogenous surfactant metabolism in rabbit lungs.
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表面活性剂治疗改变兔肺内源性表面活性剂代谢。
DOI：
10.1152/jappl.1990.68.4.1590
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发表时间：
1990
期刊：
影响因子：
--
通讯作者：
Jobe,AH
中科院分区：
文献类型：
--
作者：
Oetomo,SB;Lewis,J;Ikegami,M;Jobe,AH
使用单肺叶治疗策略评价外源性表面活性剂对内源性表面活性剂代谢的影响，以比较同一只兔中治疗与未治疗肺的影响。通过使用Swan-Ganz导管将天然兔表面活性剂Survanta或0.45%NaCl注入左主干支气管。放射性标记的棕榈酸，然后给予血管内注射两次后，表面活性剂的治疗，并在左下叶标记的掺入和分泌的比率，右肺标记的掺入和分泌进行了比较。治疗过程导致表面活性剂分布合理均匀，并且不会破坏肺叶肺血流。天然兔表面活性剂使棕榈酸盐掺入饱和磷脂酰胆碱（Sat PC）的量增加约2倍（P <0.01），经表面活性剂处理的左下叶中标记的Sat PC的分泌量增加约2.5倍（P <0.01）。虽然Survanta没有改变掺入，但它确实增加了分泌，但程度与兔表面活性剂不同（P <0.01）。内源性表面活性剂的改变在体内表面活性剂处理的Sat PC代谢是不同的，这将是由以前的体外研究预测。
The effect of exogenous surfactant on endogenous surfactant metabolism was evaluated using a single-lobe treatment strategy to compare effects of treated with untreated lung within the same rabbit. Natural rabbit surfactant, Survanta, or 0.45% NaCl was injected into the left main stem bronchus by use of a Swan-Ganz catheter. Radio-labeled palmitic acid was then given by intravascular injection at two times after surfactant treatment, and the ratios of label incorporation and secretion in the left lower lobe to label incorporation and secretion in the right lung were compared. The treatment procedure resulted in a reasonably uniform surfactant distribution and did not disrupt lobar pulmonary blood flow. Natural rabbit surfactant increased incorporation of palmitate into saturated phosphatidylcholine (Sat PC) approximately 2-fold (P less than 0.01), and secretion of labeled Sat PC increased approximately 2.5-fold in the surfactant-treated left lower lobe relative to the right lung (P less than 0.01). Although Survanta did not alter incorporation, it did increase secretion but not to the same extent as rabbit surfactant (P less than 0.01). Alteration of endogenous surfactant Sat PC metabolism in vivo by surfactant treatments was different from that which would have been predicted by previous in vitro studies.