Anti-Tumor Effect of Pinus massoniana Bark Proanthocyanidins on Ovarian Cancer through Induction of Cell Apoptosis and Inhibition of Cell Migration.
Anti-Tumor Effect of Pinus massoniana Bark Proanthocyanidins on Ovarian Cancer through Induction of Cell Apoptosis and Inhibition of Cell Migration.
复制标题
马尾松树皮原花青素通过诱导细胞凋亡和抑制细胞迁移对卵巢癌的抗肿瘤作用
DOI：
10.1371/journal.pone.0142157
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发表时间：
2015
期刊：
影响因子：
3.7
通讯作者：
Li W
中科院分区：
文献类型：
--
作者：
Liu J;Bai J;Jiang G;Li X;Wang J;Wu D;Owusu L;Zhang E;Li W
马尾松树皮原花色素（PMBPs）是从马尾松树皮中分离得到的一种活性成分，具有广泛的生物化学性质。在这里，我们研究了PMBP对卵巢癌的抗肿瘤作用。结果表明，PMBPs可显著抑制卵巢癌细胞的生长，并诱导剂量依赖性的凋亡。PMBPs可能通过抑制线粒体膜电位、下调抗凋亡蛋白Bcl-2和激活Caspase 3/9等途径诱导细胞凋亡。此外，伤口愈合和transwell小室试验显示PMBP可抑制卵巢癌细胞的迁移和侵袭。PMBPs可显著抑制MMP-9的活性和表达，阻断NFκB的活性和ERK 1/2、p38 MAPK的激活。我们的研究结果表明，PMBPs有可能被开发为卵巢癌治疗和/或疾病管理的抗肿瘤药物。
Pinus massoniana bark proanthocyanidins (PMBPs), an active component isolated from Pinus massoniana bark, has been reported to possess a wide range of biochemical properties. Here, we investigated the anti-tumor effect of PMBPs on ovarian cancer. The results indicated that PMBPs significantly reduced the growth of ovarian cancer cells and induced dose-dependent apoptosis. The underlying mechanisms involved were elucidated to include the loss of mitochondrial membrane potential, down-regulation of the anti-apoptotic protein Bcl-2 and the activation of Caspase 3/9, suggesting that PMBPs triggered apoptosis through activation of mitochondria-associated apoptotic pathway. In addition, wound healing and transwell chamber assays revealed that PMBPs could suppress migration and invasion of ovarian cancer cells. PMBPs dramatically inhibited MMP-9 activity and expression, blocked the activity of NFκB and the activation of ERK1/2 and p38 MAPK. Our findings suggest that PMBPs has the potential to be developed as an anti-tumor drug for ovarian cancer treatment and/ or disease management.