Exposure to aerosol extract from heated tobacco products causes a drastic decrease of glutathione and protein carbonylation in human lung epithelial cells
Exposure to aerosol extract from heated tobacco products causes a drastic decrease of glutathione and protein carbonylation in human lung epithelial cells
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DOI：
10.1016/j.bbrc.2021.12.004
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发表时间：
2021-12-09
影响因子：
3.1
通讯作者：
Ogasawara, Y.
中科院分区：
文献类型：
--
作者：
Nishimoto-Kusunose, S.;Sawa, M.;Ogasawara, Y.
加热烟草制品（HTPs）是一类新兴的烟草制品，声称其健康风险低于吸烟可燃烟草制品。在这项研究中，我们将人肺上皮细胞系暴露于HTP气溶胶和可燃香烟烟雾制备的提取物中，以比较细胞毒性。我们专注于醛存在于HTPs气溶胶中的影响，其水平接近于可燃香烟烟雾中的水平。证实HTP提取物具有显著毒性，尽管程度低于可燃香烟提取物。当氧化还原平衡的低分子量硫醇在细胞中的氧化损失进行评估，我们发现，总谷胱甘肽（GSH）的含量和低分子量硫醇水平显着降低后，暴露于气溶胶提取物的HTPs。这些结果表明，GSH是快速消耗过程中的外源性物质，如醛类烟草提取物的解毒。因此，暴露于HTPs的气溶胶提取物导致许多蛋白质的羰基化增强。在简单的比较中，HTPs的结果与可燃香烟烟雾的结果显著不同，表明HTPs的毒性降低。然而，我们发现在将肺上皮细胞暴露于HTPs的气溶胶提取物后，会产生显著的有害影响。因此，需要进一步的综合研究来阐明长期吸入HTPs气溶胶引起的肺损伤。(c)2021爱思唯尔公司All rights reserved.
Heated tobacco products (HTPs) are an emerging class of tobacco goods that claim to have lower health risks than those of smoking combustible tobacco products. In this study, we exposed human lung epithelial cell lines to extracts prepared from HTP aerosols and combustible cigarette smoke to compare cytotoxicity. We focused on the effects of aldehydes present in the aerosols of HTPs at levels close to those in combustible cigarette smoke. Significant toxicity was confirmed for the HTP extract, albeit to a lesser extent than that with the combustible cigarette extract. When redox balance was evaluated by the oxidative loss of low-molecular-weight thiols in the cells, we found that total glutathione (GSH) contents and low-molecular-weight thiol levels were significantly decreased after exposure to the aerosol extract of HTPs. These results indicated that GSH is rapidly consumed during the detoxification of xenobiotics, such as aldehydes from tobacco extracts. Accordingly, exposure to the aerosol extract of HTPs resulted in the enhanced carbonylation of many proteins. In a simple comparison, the results for HTPs were significantly different from those obtained with combustible cigarette smoke, suggesting reduced toxicity of HTPs. However, we found significant and harmful effects after exposing lung epithelial cells to the aerosol extract of HTPs. Thus, a further comprehensive study is needed to clarify the lung damage induced via the long-term inhalation of aerosols from HTPs. (c) 2021 Elsevier Inc. All rights reserved.