Regulation of steroidogenic acute regulatory protein expression and progesterone production in endometriotic stromal cells.
Regulation of steroidogenic acute regulatory protein expression and progesterone production in endometriotic stromal cells.
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DOI：
10.1210/jcem.86.12.8082
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发表时间：
2001-12
期刊：
影响因子：
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通讯作者：
S. Tsai;Meng-Hsing Wu;Chen-Chung Lin;H. Sun;H. Chen
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文献类型：
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作者：
S. Tsai;Meng-Hsing Wu;Chen-Chung Lin;H. Sun;H. Chen
研究了异位子宫内膜异位组织中类固醇生成急性调节蛋白（StAR）基因表达的调节以及从胆固醇合成类固醇的情况。从子宫内膜异位症患者和其他健康女性身上收集腹膜液和子宫内膜组织。与晚期子宫内膜异位症和正常女性相比，早期子宫内膜异位症的腹膜孕酮和 17β-E2 浓度最高。与腹膜类固醇的分布一致，StAR mRNA 和蛋白质在早期子宫内膜异位症的异位植入中最高。在晚期，StAR mRNA和蛋白的浓度也比正常子宫内膜中的浓度更高。相反，正常子宫内膜和异位子宫内膜异位种植体之间的 P450 侧链裂解酶和 3β-羟基类固醇脱氢酶转录本没有差异。在纯化的基质细胞而非上皮细胞中检测到 StAR mRNA 的表达。用PGE(2)而非TNFα或IL-1β治疗可显着增加StAR表达，从而诱导培养的子宫内膜异位基质细胞中孕酮的产生。这些结果表明，异位子宫内膜异位组织中StAR的异常表达导致腹膜孕酮增加与子宫内膜异位症的形成相关。腹膜PGE(2)在子宫内膜异位基质细胞中诱导StAR基因表达可能进一步促进子宫内膜异位症的发生。
The regulation of steroidogenic acute regulatory protein (StAR) gene expression and the synthesis of steroids from cholesterol in ectopic endometriosis tissues were investigated. Peritoneal fluid and endometrial tissues were collected from patients with endometriosis and otherwise healthy women. Peritoneal progesterone and 17 beta-E2 concentrations were highest in early stage endometriosis compared with those in advanced stage endometriosis and in normal women. In concordance with the profile of peritoneal steroids, StAR mRNA and protein were greatest in ectopic implants of early endometriosis. In the advanced stage, concentrations of StAR mRNA and protein were also greater compared with those in normal endometrium. In contrast, P450 side-chain cleavage enzyme and 3 beta-hydroxysteroid dehydrogenase transcripts were not different between normal endometrium and ectopic endometriotic implants. Expression of StAR mRNA was detected in purified stromal, but not epithelial, cells. Treatment with PGE(2), but not TNF alpha, or IL-1 beta significantly increased StAR expression and thus induced progesterone production in cultured endometriotic stromal cells. These results demonstrated that aberrant expression of StAR in ectopic endometriotic tissues leading to increased peritoneal progesterone is associated with the formation of endometriosis. Induction of StAR gene expression by peritoneal PGE(2) in endometriotic stromal cells may further contribute to the development of endometriosis.