Pharmacology and toxicology of pahayokolide A, a bioactive metabolite from a freshwater species of Lyngbya isolated from the Florida Everglades
Pharmacology and toxicology of pahayokolide A, a bioactive metabolite from a freshwater species of Lyngbya isolated from the Florida Everglades
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DOI：
10.1016/j.cca.2004.11.005
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发表时间：
2004-12-01
影响因子：
3.9
通讯作者：
Rein, KS
中科院分区：
文献类型：
--
作者：
Berry, JP;Gantar, M;Rein, KS
已发现丝状蓝细菌属（Lyngbya）是生物活性代谢物的丰富来源。然而，从鞘丝藻中鉴定这些化合物主要集中在少数海洋代表上。在这里，我们报告的药理学和毒理学的pahayokarma A从淡水分离，Lyngbya sp.菌株15-2，从佛罗里达大沼泽地。具体而言，我们研究了微生物代表和哺乳动物细胞系的抑制，以及化合物对无脊椎动物和脊椎动物模型的毒性。PahayoktenA抑制芽孢杆菌的代表，以及酵母，酿酒酵母。有趣的是，该化合物还抑制了几种代表性的绿色藻类，这些藻类也是从大沼泽地中分离出来的。根据细胞类型，Pahayoklastoma A在一定浓度范围内（IC 50从2.13 μ M变化到44.57 μ M）显示出抑制许多癌细胞系。当对盐水虾进行测试时，pahayoklavir在测试的最高浓度（1 mg/mL）下仅具有轻微毒性。然而，该化合物对斑马鱼胚胎有急性毒性（LC 50 =2.15 μ M）。可能的生物医学和环境健康方面的pahayokolides仍有待调查，然而，生物活性代谢物的鉴定，如这些证明了潜在的佛罗里达大沼泽地作为新的毒素和药物的来源。(C)2004年爱思唯尔公司All rights reserved.
The genus of filamentous cyanobacteria, Lyngbya, has been found to be a rich source of bioactive metabolites. However, identification of such compounds from Lyngbya has largely focused on a few marine representatives. Here, we report on the pharmacology and toxicology of pahayokolide A from a freshwater isolate, Lyngbya sp. strain 15-2, from the Florida Everglades. Specifically, we investigated inhibition of microbial representatives and mammalian cell lines, as well as toxicity of the compound to both invertebrate and vertebrate models. Pahayokolide A inhibited representatives of Bacillus, as well as the yeast, Saccharomyces cerevisiae. Interestingly, the compound also inhibited several representatives of green algae that were also isolated from the Everglades. Pahayokolide A was shown to inhibit a number of cancer cell lines over a range of concentrations (IC50 varied from 2.13 to 44.57 muM) depending on the cell-type. When tested against brine shrimp, pahayokolide was only marginally toxic at the highest concentrations tested (1 mg/mL). The compound was, however, acutely toxic to zebrafish embryos (LC50=2.15 muM). Possible biomedical and environmental health aspects of the pahayokolides remain to be investigated; however, the identification of bioactive metabolites such as these demonstrates the potential of the Florida Everglades as source of new toxins and drugs. (C) 2004 Elsevier Inc. All rights reserved.