SULT1E1 modified the association between phytoestrogen consumption and bone mineral density in healthy Korean women
SULT1E1 modified the association between phytoestrogen consumption and bone mineral density in healthy Korean women
复制标题
DOI：
10.1007/s00223-006-0008-4
复制
发表时间：
2006-09-01
影响因子：
4.2
通讯作者：
Kang, D.
中科院分区：
文献类型：
--
作者：
Lee, S. A.;Choi, J. Y.;Kang, D.
磺基转移酶1 E1（Sulfotransferase 1 E1，SULT 1 E1）催化雌激素转化为硫酸盐结合，参与植物雌激素的代谢。对397名韩国妇女进行了一项以社区为基础的横断面研究，以评估SULT 1 E1基因多态性与骨密度（BMD）之间的关联，以及韩国妇女BMD的基因多态性和植物雌激素摄入量的综合效应。采用双能X线骨密度仪测量桡骨远端和跟骨的BMD。通过5 '-核酸酶测定（TaqMan）确定SULT 1 E1 IVS 1 -447 C > A、IVS 4 -1653 T > C和 *959 G > A的基因型。植物雌激素的摄入量是通过食物频率问卷来评估的，该问卷是根据多次24小时召回进行验证的。与AA基因型相比，SULT 1 E1 *959 GG基因型女性桡骨远端BMD降低4.5%（P趋势= 0.05），跟骨BMD降低7.9%（P趋势< 0.01），而SULT 1 E1 IVS 1 -447 CC基因型和IVS 4 -1653 TT基因型与BMD无关。跟骨骨密度随CTG单倍型的增加无明显变化趋势，但SULT 1 E1 CTA-CTA单倍型与CTG-CCA单倍型跟骨骨密度差异有统计学意义（P < 0.05）。当按SULT 1 E1基因型分层时，植物雌激素消耗量与跟骨BMD之间的相关性在SULT 1 E1 *959 GG基因型（r = 0.25，P = 0.01）或SULT 1 E1 IVS 4-1653 TT基因型（r = 0.15，P = 0.02）的女性中值得注意。在用错误发现率方法校正多重检验后，这种趋势仅在绝经后妇女中保持显著性（r = 0.36，P = 0.01）。总之，SULT 1 E1 *959 G > A基因多态性与桡骨远端和跟骨的BMD相关，植物雌激素消耗量与跟骨BMD之间的关联可能被该基因多态性所改变。
Sulfotransferase 1E1 (SULT1E1) catalyze estrogen into sulfate conjugation and is involved in the metabolism of phytoestrogen. A community-based cross-sectional study was conducted on 397 Korean women, to evaluate the association between genetic polymorphisms of SULT1E1 and bone mineral density (BMD) and the combined effect of the genetic polymorphism and phytoestrogen intake for BMD in Korean women. BMDs of the distal radius and the calcaneus were measured by dual-energy X-ray absorptiometry. Genotypes of SULT1E1 IVS1-447 C > A, IVS4-1653 T > C, and *959 G > A were determined by the 5'-nuclease assay (TaqMan). Phytoestrogen intake was estimated by a food-frequency questionnaire validated against multiple 24-hour recalls. Women with the SULT1E1 *959 GG genotype had a 4.5% lower BMD at the distal radius (P-trend = 0.05) and a 7.9% lower BMD at the calcaneus compared to those with AA genotype (P-trend < 0.01), whereas the SULT1E1 IVS1-447 CC genotype and IVS4-1653 TT genotype were not associated with BMD. There was no significant trend of BMD with the numbers of CTG-containing haplotypes, but calcaneal BMDs significantly differed between SULT1E1 CTA-CTA haplotype and CTG-CCA haplotype (P < 0.05). When stratified by SULT1E1 genotype, the correlation between phytoestrogen consumption and BMD at the calcaneus was noteworthy in women with SULT1E1 *959 GG genotype (r = 0.25, P = 0.01) or SULT1E1 IVS 4-1653 TT genotype (r = 0.15, P = 0.02). This trend remained significant only in post-menopausal women (r = 0.36, P = 0.01) after multiple testing was corrected by the false discovery rate method. In conclusion, the genetic polymorphism of SULT1E1 *959 G > A was associated with BMD at the distal radius and calcaneus, and the association between phytoestrogen consumption and calcaneal BMD might be modified by this genetic polymorphism.