Molecular characterization and phylogenetic analysis of the reticuloendotheliosis virus isolated from wild birds in Northeast China
Molecular characterization and phylogenetic analysis of the reticuloendotheliosis virus isolated from wild birds in Northeast China
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DOI：
10.1016/j.vetmic.2013.05.008
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发表时间：
2013-09-27
影响因子：
3.3
通讯作者：
Wang, Xiaomei
中科院分区：
文献类型：
--
作者：
Jiang, Lili;Qi, Xiaole;Wang, Xiaomei
为分析我国野生鸟类网状内皮病（RE）感染状况，对辽宁、吉林、黑龙江三省采集的585份野生鸟类样本进行了调查分析。采样的鸟类代表 3 个目、40 多个物种。病毒分离和PCR扩增表明，部分野鸟感染了REV，共分离出10株REV。对 10 个 REV 毒株中每一个毒株的 gp90 基因进行了扩增、克隆和测序。序列分析表明，本研究分离的10株REV毒株的gp90基因在核苷酸水平上与中国东北毒株HLJR0901和HLJR0801以及在美国和台湾发现的一些REV毒株比与中国早期REV毒株HA9901更加相似。此外，系统发育分析表明，10 个 REV 毒株的 gp90 基因与 REV 亚型 III 代表毒株（CSV）比与毒株 170A（I 亚型）或 SNV（II 亚型）更相似。这是第一项调查感染 REV 的野生鸟类状况的研究。本文的研究结果不仅将为进一步了解REV的进化提供必要的信息，而且还确定了野生鸟类在REV传播中的潜在作用，并进一步加深了我们对野生鸟类REV生态学的理解。 (C) 2013 Elsevier B.V. 保留所有权利。
To analyze the status of reticuloendotheliosis (RE) infection of wild birds in China, 585 samples from wild birds collected in Liaoning, Jilin and Heilongjiang provinces China were investigated and analyzed. The sampled birds represent 3 orders and more than 40 species. Virus isolation and PCR amplification showed that some of the wild birds were infected with REV, and 10 REV strains were isolated. The gp90 gene from each of the 10 REV strains was amplified, cloned, and sequenced. Sequence analysis indicated that the gp90 genes of the 10 REV strains isolated in this study were more similar at the nucleotide level with the northeast Chinese strains HLJR0901 and HLJR0801 and some REV strains found in the US and Taiwan than with the early Chinese REV isolate HA9901. Furthermore, phylogenetic analysis indicated that the gp90 genes of the 10 REV strains were more similar to the REV subtype III-representing strain (CSV) than to strains 170A (subtype I) or SNV (subtype II). This is the first study to investigate the status of wild birds infected with REV. The results of this paper will not only provide necessary information for further understanding the evolution of REV, but they also identify the potential role of wild birds in REV transmission and furthers our understanding of the ecology of REV in wild bird species. (C) 2013 Elsevier B.V. All rights reserved.