CD4+CD25high regulatory T cells are markedly decreased in blood of patients with pemphigus vulgaris
CD4+CD25high regulatory T cells are markedly decreased in blood of patients with pemphigus vulgaris
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DOI：
10.1159/000099585
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发表时间：
2007-01-01
期刊：
影响因子：
3.4
通讯作者：
Shimada, S.
中科院分区：
文献类型：
--
作者：
Sugiyama, H.;Matsue, H.;Shimada, S.
背景：尚不清楚寻常型天疱疮（PV），一种自身免疫性水疱疾病，是否与CD4(+)CD25（高）调节性T （Treg）细胞减少和/或功能障碍有关。目的：评价PV患者血液中Treg细胞的频率和表型。方法：制备PV患者及正常、对照志愿者外周血单个核细胞，流式细胞术检测Treg细胞的频率和表型。采用实时半定量RT-PCR技术检测PV患者和正常人外周血单个核细胞CD4(+)CD25（+）和CD4(+)CD25- t细胞Foxp3基因的表达。结果：所有PV患者的Treg细胞比例严重降低，约为对照组的10倍。在PV患者CD4(+)CD25(+) T细胞群中Foxp3基因和蛋白表达的减少进一步证实了这些观察结果。结论：Treg细胞的数值损伤可能参与了PV的发病机制。版权所有(c) 2007 S. Karger AG，巴塞尔。
Background: It remains to be determined whether pemphigus vulgaris (PV), an autoimmune blistering disease, has a reduction and/ or dysfunction of CD4(+)CD25(high) regulatory T (Treg) cells. Objectives: To evaluate the frequency and phenotypes of Treg cells in blood of patients with PV. Methods: Peripheral blood mononuclear cells were prepared from PV patients as well as normal and disease control volunteers, and the frequency and phenotypes of Treg cells were determined by flow cytometry. CD4(+)CD25(+) and CD4(+)CD25-T cells isolated from peripheral blood mononuclear cells of PV patients and normal controls were subjected to real-time semiquantitative RT-PCR for the expression of Foxp3 gene. Results: The proportion of Treg cells in all PV patients was severely reduced, approximately ten times less than controls. These observations were further confirmed by both diminished gene and protein expression of Foxp3 in the CD4(+)CD25(+) T cell population in PV patients. Conclusions: Numerical impairment of Treg cells may be involved in the pathogenesis of PV. Copyright (c) 2007 S. Karger AG, Basel.