Comparison of virus shedding after lived attenuated and pentavalent reassortant rotavirus vaccine
Comparison of virus shedding after lived attenuated and pentavalent reassortant rotavirus vaccine
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DOI：
10.1016/j.vaccine.2013.08.041
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发表时间：
2014-02-26
期刊：
影响因子：
5.5
通讯作者：
Huang, Yhu-Chering
中科院分区：
文献类型：
--
作者：
Hsieh, Yu-Chia;Wu, Fang-Tzy;Huang, Yhu-Chering
有报告称轮状病毒疫苗或疫苗抗性株传播给未接种疫苗的接触者。因此，有必要评估和描述轮状病毒疫苗接种者中疫苗病毒脱落的性质。两组接受完整疗程RotaTeq（RV 5）或Rotarix（RV 2）的健康婴儿入组（2010年3月至2011年6月），以比较每次疫苗接种后1个月的粪便脱落。使用酶免疫测定法（EIA）和实时逆转录-聚合酶链反应（RT-PCR）评估脱落。87名婴儿（34名女孩和53名男孩）入组研究。首剂疫苗接种后，第4 ~ 7天为病毒散毒高峰期，实时荧光定量RT-PCR阳性检出率为80-90%，EIA阳性检出率为20-30%。在两组中，疫苗脱落最早发生在1天，最晚发生在25-28天。实时RT-PCR数据的混合效应logistic回归分析显示，当比较第一次疫苗接种后（比值比[OR] 1.26; P=0.71）或第二次疫苗接种后（比值比[OR] 1.26; P=0.99）的脱落率时，两组之间无显著差异。然而，在第一次疫苗接种后（P=0.001）和第二次疫苗接种后（P=0.039），接受RV 2的婴儿的病毒载量显著高于接受RV 5的婴儿。在通过实时RT-PCR检测的脱落率方面，台湾婴儿对RV 5或RV 2的疫苗摄取是相当的。RV 2中较高的脱落病毒载量的临床意义应进一步观察。(C)2014爱思唯尔有限公司版权所有。
Transmission of rotavirus vaccine or vaccine-reassortant strains to unvaccinated contacts has been reported. Therefore, it is essential to evaluate and characterize the nature of vaccine-virus shedding among rotavirus vaccine recipients. Two groups of healthy infants who received a complete course of RotaTeq (RV5) or Rotarix (RV2) were enrolled (between March 2010 and June 2011) to compare fecal shedding for one month after each vaccine dose. Shedding was assessed using both enzyme immunoassay (EIA) and real-time reverse transcription-polymerase chain reaction (RT-PCR). Eighty-seven infants (34 girls and 53 boys) were enrolled in the study. After the first vaccine dose, the peak time of virus shedding occurred between day 4 and day 7, with positive detection rates of 80-90% by real-time RT-PCR and 20-30% by EIA. In both groups, vaccine shedding occurred as early as one day and as late as 25-28 days. Mixed effects logistic regression analysis of real-time RT-PCR data showed no significant differences between two groups when shedding rates were compared after the first vaccine dose (odds ratio [OR] 1.26; P=0.71) or after the second vaccine dose (odds ratio [OR] 1.26; P=0.99). However, infants receiving RV2 shed significantly higher viral loads than those receiving RV5 when compared after the first vaccine dose (P=0.001) and after the second dose (P=0.039). In terms of shedding rates detected by real-time RT-PCR, vaccine uptake of RV5 or RV2 among infants in Taiwan was comparable. Clinical significance of higher shedding viral loads in RV2 should be further observed. (C) 2014 Elsevier Ltd. All rights reserved.