Effects of electroacupuncture on cold allodynia in a rat model of neuropathic pain: Mediation by spinal adrenergic and serotonergic receptors
Effects of electroacupuncture on cold allodynia in a rat model of neuropathic pain: Mediation by spinal adrenergic and serotonergic receptors
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DOI：
10.1016/j.expneurol.2005.06.018
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发表时间：
2005-10-01
影响因子：
5.3
通讯作者：
Na, HS
中科院分区：
文献类型：
--
作者：
Kim, SK;Park, JH;Na, HS
本研究在神经病理性疼痛的大鼠模型上，观察了电针治疗冷性超敏的作用及其与脊髓肾上腺素能和5-羟色胺能系统的关系。神经外科手术中，在支配尾部的S1、S2脊神经之间切除右侧尾部上干。神经损伤后2周，电针刺激足三里穴(2或100 Hz)30min。刺激前后用浸尾试验[即将尾巴浸入4℃冷水中，测量尾巴突然移动的潜伏期]来评价冷超敏的行为体征。然后，我们观察鞘内注射哌唑嗪(α(1)受体拮抗剂，30mU g)、育亨宾(α(2)肾上腺素受体拮抗剂，30mU g)、NaN-190(5-HT1a拮抗剂，15mU g)、酮丝林(5-HT2a拮抗剂，30mU g)和MDL-72222(5-HT3拮抗剂，12mU g)对电针刺激作用的影响。虽然2赫兹和100赫兹电针均能显著缓解冷刺激引起的痛觉过敏症状，但2赫兹电针的作用比100赫兹电针更强。此外，鞘内注射育亨宾、NaN190和MDL-72222，但不阻断哌唑嗪和酮色林，可显著阻断2 Hz电针对冷性超敏的缓解作用。这些结果表明，低频(2 Hz)电针比高频(100 Hz)电针更适合治疗冷性痛觉超敏，而脊髓α(2)-肾上腺素能、5-HT1a和5-HT3受体在介导2 Hz电针缓解神经性大鼠冷性痛觉过敏中起重要作用。(C)2005 Elsevier Inc.保留所有权利。
The present study was performed to examine the effects of electroacupuncture (EA) on cold allodynia and its mechanisms related to the spinal adrenergic and serotonergic systems in a rat model of neuropathic pain. For the neuropathic surgery, the right superior caudal trunk was resected at the level between S1 and S2 spinal nerves innervating the tail. Two weeks after the nerve injury, EA stimulation (2 or 100 Hz) was delivered to Zusanli (ST36) for 30 min. The behavioral signs of cold allodynia, were evaluated by the tail immersion test [i.e., immersing the tail in cold water (4 degrees C) and measuring the latency to an abrupt tail movement] before and after the stimulation. And then, we examined the effects of intrathecal injection of prazosin (alpha(1)-adrenoceptor antagonist, 30 mu g), yohimbine (alpha(2)-adrenoceptor antagonist, 30 mu g), NAN-190 (5-HT1A antagonist, 15 mu g), ketanserin (5-HT2A antagonist, 30 mu g), and MDL-72222 (5-HT3 antagonist, 12 mu g) on the action of EA stimulation. Although both 2 Hz and 100 Hz EA significantly relieved the cold allodynia signs, 2 Hz EA induced more robust effects than 100 Hz EA. In addition, intrathecal injection of yohimbine, NAN-190, and MDL-72222, but not prazosin and ketanserin, significantly blocked the relieving effects of 2 Hz EA on cold allodynia. These results suggest that low-frequency (2 Hz) EA is more suitable for the treatment of cold allodynia than high-frequency (100 Hz) EA, and spinal alpha(2)-adrenergic, 5-HT1A and 5-HT3, but not alpha(1)-adrenergic and 5-HT2A, receptors play important roles in mediating the relieving effects of 2 Hz EA on cold allodynia in neuropathic rats. (c) 2005 Elsevier Inc. All rights reserved.