Morphometric analysis in potentially malignant head and neck lesions: oral submucous fibrosis.
Morphometric analysis in potentially malignant head and neck lesions: oral submucous fibrosis.
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发表时间：
2010
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通讯作者：
Mamta Singh;A. Chaudhary;S. Pandya;S. Debnath;Mangal Singh;PA Singh;R. Mehrotra
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作者：
Mamta Singh;A. Chaudhary;S. Pandya;S. Debnath;Mangal Singh;PA Singh;R. Mehrotra
目的：本研究的目的是分析口腔粘膜下纤维性变（OSMF）I、II、III和IV级病例的上皮、血管和纤维化的形态计量学，并确定Pindborg和Sirsat后组织学分级的任何相关性。材料与方法83例口腔粘膜下纤维性变病例在印度阿拉哈巴德的M.L.N医学院病理学系使用交互式图像分析系统进行形态计量学分析。石蜡包埋的3-4 μ m厚切片用苏木精/伊红、货车Gieson苦味酸和酸性品红染色和Masson三色染色。使用特定软件（Image Pro Plus 6.0）进行图像分析，并将获得的数据最终传输到Excel表中，用于计算每个参数的平均值。结果按分级标准，OSMF Ⅰ级9例，Ⅱ级32例，Ⅲ级39例，Ⅳ级3例。临床牙关紧闭最常见于IV级，其次分别为III级、II级和I级。OSMF I级病例未显示任何可测量的胶原化量，而OSMF I级和II级[Pearson卡方检验= 85.72; p= 0.051]以及OSMF-III级和IV级[Pearson卡方检验=188.74; p<0.001]显示胶原化显著增加。低倍视野下的内皮细胞数随级别的增加而减少。结论：平均血管面积和平均血管直径在II级中显著增加，而在IV级和III级中显著减少，胶原厚度（μ m）随着级别的增加而增加，而内皮细胞密度降低。
OBJECTIVE The objective of this study is to analyze cases of oral submucous fibrosis (OSMF) Grade I, II, III and IV morphometrically with regard to epithelium, vasculature and fibrosis and determine any correlation with histological grading after Pindborg and Sirsat. MATERIALS AND METHODS Eighty three oral submucous fibrosis cases were analyzed morpometrically using an interactive image analysis system in the Department of Pathology, M.L.N Medical College, Allahabad, U.P, India. Paraffin embedded sections of 3-4 microm thickness were stained with hematoxylin/eosin, Van Gieson's picric acid and acid fuchsin stain and Masson's trichrome stains. Image analysis was performed with specific software (Image Pro Plus 6.0) and data obtained were finally transported to Excel sheets for calculation of average values for each parameter. RESULTS With the grading criteria applied, 9 cases of OSMF were grade I, 32 grade II, 39 grade III and 3 grade IV. Clinical trismus was most frequent in Grade IV followed by Grade III, II and I respectively. OSMF Grade I cases did not show any measurable amount of collagenization, whereas it showed a significant increase with OSMF I and II grades [Pearson's chi2 test= 85.72; p= 0.051] and OSMF-III and IV [Pearson's chi2 test=188.74; p<0.001]. Numbers of endothelial cells per low power field consistently decreased with the increasing grade. CONCLUSIONS We concluded that mean blood vessel area and the mean vessel diameter showed a marked increase in grade II and a marker decrease in grade IV and the grade III, collagen thickness (mum) increases according to increasing grade while density of endothelial cells decreases.