Whole-genome resequencing of extreme phenotypes in collared flycatchers highlights the difficulty of detecting quantitative trait loci in natural populations
Whole-genome resequencing of extreme phenotypes in collared flycatchers highlights the difficulty of detecting quantitative trait loci in natural populations
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DOI：
10.1111/1755-0998.12498
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发表时间：
2016-05-01
影响因子：
7.7
通讯作者：
Ellegren, Hans
中科院分区：
文献类型：
--
作者：
Kardos, Marty;Husby, Arild;Ellegren, Hans
剖析自然种群表型变异的遗传基础是进化生物学长期追求的目标。一个悬而未决的问题是，数量性状是只由大量效应较小的基因决定的，还是大效应基因座也存在变异。我们对81只具有极端表型的全基因组重新测序的雄性捕蝇者和415只雄性捕蝇者进行了前额斑块大小(性别选择性状)的全基因组关联分析，这些雄性斑块大小与斑块大小无关，并用50K SNP芯片进行了基因分型。在全基因组范围内，没有SNP与斑块大小有统计学意义的关联。基于模拟的功率分析表明，对10%的表型变异负责的大效应基因座的检测能力是
Dissecting the genetic basis of phenotypic variation in natural populations is a long-standing goal in evolutionary biology. One open question is whether quantitative traits are determined only by large numbers of genes with small effects, or whether variation also exists in large-effect loci. We conducted genomewide association analyses of forehead patch size (a sexually selected trait) on 81 whole-genome-resequenced male collared flycatchers with extreme phenotypes, and on 415 males sampled independent of patch size and genotyped with a 50K SNP chip. No SNPs were genomewide statistically significantly associated with patch size. Simulation-based power analyses suggest that the power to detect large-effect loci responsible for 10% of phenotypic variance was