Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization.
Developmental expression of 2ar (osteopontin) and SPARC (osteonectin) RNA as revealed by in situ hybridization.
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DOI：
10.1083/jcb.106.2.441
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发表时间：
1988-02
期刊：
影响因子：
--
通讯作者：
Hogan BL
中科院分区：
文献类型：
--
作者：
Nomura S;Wills AJ;Edwards DR;Heath JK;Hogan BL
2ar已被鉴定为在多种培养的小鼠细胞系中可由肿瘤启动子和生长因子诱导的基因（Smith，J.H.，和D. T.登哈特1987. J.细胞。34：13-22）。序列分析显示其编码小鼠骨桥蛋白，一种最初从骨分离的含RGDS的磷酸化的富含唾液酸的Ca++结合蛋白（Oldberg，A.，A. Franzen和D.海涅加德1986. Proc. Natl. Acad. Sci. USA. 83：8819-8823; Prince，C. W.，T.大泽湾T.巴特勒，M。Tomana，A. S. Brown和R. E.施罗亨洛1987. J.Biol.Chem.262：2900-3907）。本文应用北方印迹分析和原位杂交技术对小鼠胚胎发育过程中2ar的表达进行了定位。2ar RNA在出生后14.5 d首次在发育中的肢骨和颅骨中检测到，在不同于表达骨粘连蛋白（osteonectin）的细胞群中。高水平的2ar表达也见于骨髓来源的颗粒状子宫腺细胞的蜕膜和胎盘，并在一些上皮组织，包括胚胎和出生后的肾小管，子宫上皮和感觉上皮的胚胎耳。在体内观察到的2ar表达的时间和空间模式表明，该蛋白质在不局限于骨发育的过程中起着比以前认识到的更广泛的作用。
2ar has been identified as a gene inducible by tumor promoters and growth factors in a variety of cultured mouse cell lines (Smith, J. H., and D. T. Denhardt. 1987. J. Cell. Biochem. 34:13-22). Sequence analysis shows that it codes for mouse osteopontin, an RGDS-containing, phosphorylated, sialic acid-rich Ca++-binding protein originally isolated from bone (Oldberg, A., A. Franzen, and D. Heinegard. 1986. Proc. Natl. Acad. Sci. USA. 83:8819-8823; Prince, C. W., T. Oosawa, W. T. Butler, M. Tomana, A. S. Brown, and R. E. Schrohenloer. 1987. J. Biol. Chem. 262:2900-3907.). In this paper we use Northern blot analysis and in situ hybridization to localize expression of 2ar during mouse embryogenesis. 2ar RNA is first detected in developing limb bones and calvaria at 14.5 d p.c., in a population of cells distinct from those expressing SPARC (osteonectin). High levels of 2ar expression are also seen in the bone marrow-derived granulated metrial gland cells of the deciduum and placenta, and in a number of epithelial tissues, including embryonic and postnatal kidney tubules, uterine epithelium and sensory epithelium of the embryonic ear. The temporal and spatial pattern of 2ar expression seen in vivo suggests that the protein plays a wider role than previously realized, in processes which are not confined to bone development.