Intragastric administration of allyl isothiocyanate increases carbohydrate oxidation via TRPV1 but not TRPA1 in mice
Intragastric administration of allyl isothiocyanate increases carbohydrate oxidation via TRPV1 but not TRPA1 in mice
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DOI：
10.1152/ajpregu.00645.2009
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发表时间：
2011-06-01
影响因子：
2.8
通讯作者：
Fushiki, Tohru
中科院分区：
文献类型：
--
作者：
Mori, Noriyuki;Kawabata, Fuminori;Fushiki, Tohru
[10] Mori N，Kawabata F，Matsumura S，Hosokawa H，小林S，Inoue K，Fushiki T.异硫氰酸烯丙酯的胃内给药增加小鼠中通过TRPV 1而不是TRPA 1的碳水化合物氧化。Am J Physiol Regul Integr Comp Physiol 300：R1494-R1505，2011.首次发表于2011年3月23日; doi：10.1152/ajpregu.00645.2009。瞬时受体电位（TRP）通道家族是由各种阳离子渗透性通道组成的，这些通道被各种刺激多模式激活，并参与多种细胞功能。最近的研究表明，TRP通道的激活不仅参与伤害感受和温度感觉，而且参与体温调节和能量代谢。我们研究了灌胃给予TRP通道激动剂对小鼠能量底物利用变化的影响。异硫氰酸烯丙酯（AITC;一种典型的TRPA 1激动剂）的胃内给药显著增加碳水化合物氧化，但不影响氧消耗。为了研究TRP通道是否介导碳水化合物氧化的增加，我们使用TRPA 1和TRPV 1敲除（KO）小鼠。AITC胃内给药增加TRPA 1 KO小鼠的碳水化合物氧化，但在TRPV 1 KO小鼠中不增加。此外，AITC剂量依赖性地增加表达TRPV 1的细胞中的细胞内钙离子浓度。这些发现表明，AITC可能激活TRPV 1，并且AITC通过TRPV 1增加碳水化合物氧化。
Mori N, Kawabata F, Matsumura S, Hosokawa H, Kobayashi S, Inoue K, Fushiki T. Intragastric administration of allyl isothiocyanate increases carbohydrate oxidation via TRPV1 but not TRPA1 in mice. Am J Physiol Regul Integr Comp Physiol 300: R1494-R1505, 2011. First published March 23, 2011; doi:10.1152/ajpregu.00645.2009.-The transient receptor potential (TRP) channel family is composed of a wide variety of cation-permeable channels activated polymodally by various stimuli and is implicated in a variety of cellular functions. Recent investigations have revealed that activation of TRP channels is involved not only in nociception and thermosensation but also in thermoregulation and energy metabolism. We investigated the effect of intragastric administration of TRP channel agonists on changes in energy substrate utilization of mice. Intragastric administration of allyl isothiocyanate (AITC; a typical TRPA1 agonist) markedly increased carbohydrate oxidation but did not affect oxygen consumption. To examine whether TRP channels mediate this increase in carbohydrate oxidation, we used TRPA1 and TRPV1 knockout (KO) mice. Intragastric administration of AITC increased carbohydrate oxidation in TRPA1 KO mice but not in TRPV1 KO mice. Furthermore, AITC dose-dependently increased intracellular calcium ion concentration in cells expressing TRPV1. These findings suggest that AITC might activate TRPV1 and that AITC increased carbohydrate oxidation via TRPV1.