Respiratory syncytial virus infection up-regulates TLR7 expression by inducing oxidative stress via the Nrf2/ARE pathway in A549 cells
Respiratory syncytial virus infection up-regulates TLR7 expression by inducing oxidative stress via the Nrf2/ARE pathway in A549 cells
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呼吸道合胞病毒感染通过 Nrf2/ARE 途径诱导 A549 细胞氧化应激上调 TLR7 表达
DOI：
10.1007/s00705-018-3739-4
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发表时间：
2018-02
影响因子：
2.7
通讯作者：
Sheng-hai Huang
中科院分区：
文献类型：
--
作者：
Tao Sun;Hai-yang Yu;Chuan-long Zhang;Tong-na Zhu;Sheng-hai Huang
为了更好地理解RSV的发病机制和对RSV的免疫应答的早期途径，本文中，我们探索了A549细胞中RSV感染后TLR 7表达和氧化应激诱导之间的关系。我们研究了Nrf 2/ARE通路激动剂丁基羟基茴香醚（BHA）和抑制剂胡芦巴碱（TRI）对TLR 7调节或氧化应激诱导的干预作用。为了比较，我们在本研究中设置了7个处理组，包括RSV处理的细胞、BHA + RSV处理的细胞、TRI + RSV处理的细胞、正常细胞对照、灭活RSV对照、BHA对照和TRI对照。用RT-PCR法检测TLR 7、IL-6、TNF-α mRNA的表达，ELISA法检测IL-6、TNF-α、IL-1β蛋白的表达，Western blot法检测TLR 7、Nrf 2、HO-1蛋白的表达。我们还评估了细胞增殖的变化，并测量了不同处理组A549细胞中·OH和NO的变化。结果表明，TLR 7的上调与RSV感染和氧化应激的诱导有关，并且TLR 7的表达由Nrf 2/ARE通路抑制剂或激动剂的抗炎作用介导。我们的实验可能有助于阐明RSV感染的潜在病理学，并为药物开发和预防RSV诱导的人类疾病提供潜在的治疗靶点。
In order to better understand the early pathways of the pathogenesis of, and immune response to, RSV, herein, we explored the relationship between TLR7 expression and oxidative stress induction following RSV infection in A549 cells. We studied the intervening effects of the Nrf2/ARE pathway agonist butylated hydroxyanisole (BHA) and inhibitor trigonelline (TRI) on TLR7 modulation or oxidative stress induction. For comparison purposes, we set up seven treatment groups in this study, including RSV-treated cells, BHA + RSV-treated cells, TRI + RSV-treated cells, normal cell controls, inactivated RSV controls, BHA controls and TRI controls. We measured changes in TLR7, IL-6, TNF-α mRNA using RT-PCR and IL-6, TNF-α and IL-1β protein using ELISA as well as TLR7, Nrf2 and HO-1 protein using Western blot in A549 cells from the different treatment groups. We also assessed changes in cell proliferation and measured changes in ·OH and NO in A549 cells from the different treatment groups. The results indicate that TLR7 up-regulation is related to RSV infection and the induction of oxidative stress and that TLR7 expression was mediated by the anti-inflammatory effects of Nrf2/ARE pathway inhibitors or agonists. Our experiments may help elucidate the underlying pathology of RSV infection and suggest potential therapeutic targets for drug development and the prevention of RSV-induced human diseases.
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影响因子：
3.7
作者：
Ivanov AV;Smirnova OA;Ivanova ON;Masalova OV;Kochetkov SN;Isaguliants MG
通讯作者：
Isaguliants MG
DOI：
10.1164/rccm.200804-535oc
发表时间：
2009-01-15
影响因子：
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影响因子：
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作者：
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影响因子：
7.4
作者：
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通讯作者：
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