Retention of ionizable compounds on HPLC.: 8.: Influence of mobile-phase pH change on the chromatographic retention of acids and bases during gradient elution
Retention of ionizable compounds on HPLC.: 8.: Influence of mobile-phase pH change on the chromatographic retention of acids and bases during gradient elution
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DOI：
10.1021/ac0101454
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发表时间：
2001-10-15
影响因子：
7.4
通讯作者：
Rosés, M
中科院分区：
文献类型：
--
作者：
Canals, I;Valkó, K;Rosés, M
研究了酸碱在梯度洗脱中的保留率与流动相pH的关系。从较宽的pH值范围内测定了乙腈和甲醇用量增加引起的表观pH漂移。结果表明，如果对所有的pH值都使用相同类型的缓冲液(在本工作中为醋酸铵)，则可电离化合物的保留率与水缓冲液的pH之间可以建立良好的关系。提出了将梯度保留数据与水缓冲液的pH进行拟合的方程。Ile提出的方程解释了在梯度洗脱过程中，随着缓冲液pH的变化，化合物的pK(A)的相对变化。
The relationships between retention and mobile-phase pH in gradient elution are studied for acids and bases. The apparent pH shift caused by the increasing amount of acetonitrile and methanol has been determined starting from a wide range of pH values. It is shown that good relationships between the retention of ionizable compounds and the pH of the aqueous buffer can be established if the same type of buffer (ammonium acetate in this work) is used for all pH points. Equations are proposed to fit the gradient retention data to, the pH of the aqueous buffer. Ile proposed equation gives an account of the relative variation of the pK(a) of the compound in the reference to the variation of the pH of the buffer as both parameters change during gradient elution.