Comparison between the nitric oxide reductase family and its aerobic relatives, the cytochrome oxidases
Comparison between the nitric oxide reductase family and its aerobic relatives, the cytochrome oxidases
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DOI：
10.1042/bst0300662
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发表时间：
2002-08-01
影响因子：
3.9
通讯作者：
Schröder, I
中科院分区：
文献类型：
--
作者：
de Vries, S;Schröder, I
研究了高温古菌Pyrobaculum aerophilum的反硝化途径。与革兰氏阴性菌相比，所有四种反硝化酶都是膜结合的。嗜气假单胞菌也是迄今为止鉴定的唯一一种其中甲基喹啉是所有四种反硝化还原酶的电子供体的假单胞菌。嗜气假单胞菌的NO还原酶（NOR）属于血红素铜氧化酶超家族，并且是qNOR（喹啉依赖性）类型。迄今为止，已经纯化了三种类型的NOR：cNOR（细胞色素c/假天青不依赖性）、qNOR和qCu（A）NOR（在电子进入位点含有Cu-A的qNOR）。有人建议，NORs和各种细胞色素氧化酶的模块化进化，鉴于其电子供体位点的结构。qNOR进一步被认为是属于haern-copper oxidases超家族的所有NOR和cvtochrome oxidases的祖先。
The denitrification pathway has been studied in the hyperthermophilic archaeon Pyrobaculum aerophilum. In contrast with Gram-negative bacteria, all four denitrification enzymes are membrane-bound. P. aerophilum is also the only denitrifyer identified so far in which menaquinol is the electron donor to all four denitrification reductases. The NO reductase NOR) of P. aerophilum belongs to the superfamlily of haem-copper oxidases and is of the qNOR (quinol-dependent) type. Three types of NOR have been purified so far: cNOR (cytochrome c/pseudoazurindependent), qNOR and qCu(A)NOR (qNOR that contains Cu-A at the electron entry site). It is proposed that the NORs and the various cytochrome oxidases have evolved by modular evolution, in view of the structure of their electron donor sites. qNOR is further proposed to be the ancestor of all NORs and cvtochrome oxidases belonging to the superfamily of haern-copper oxidases.