Rational construction of an AIE-active fluorescent probe bearing three reaction sites for individual and continuous detection of H2S and HClO with single-wavelength excitation
Rational construction of an AIE-active fluorescent probe bearing three reaction sites for individual and continuous detection of H2S and HClO with single-wavelength excitation
复制标题
合理构建具有三个反应位点的 AIE 活性荧光探针，用于单波长激发下单独连续检测 H2S 和 HClO
DOI：
10.1016/j.snb.2022.132900
复制
发表时间：
2022-10-31
影响因子：
8.4
通讯作者：
Zhang, Youyu
中科院分区：
文献类型：
--
作者：
Gu, Biao;Dai, Cong;Zhang, Youyu
内源性H_2S和HClO在体内氧化还原状态的调节中起着至关重要的作用，并参与许多病理生理事件。因此，对细胞内H_2S和HClO的准确传感和成像就变得非常迫切。然而，传统的双反应荧光探针对这两种物质都存在聚集猝灭效应，需要多次激发才能实现分辨测定。在这项研究中，我们首先构建了一个具有三个潜在反应位点的AIE活性荧光探针，用于使用单一激发波长单独和连续检测H_2S和HClO。该多位探针对H_2S和HClO表现出独特的荧光行为，响应速度快(H_2S：12min；HCl：1.8min)，发射峰分离良好(88 Nm)，由于H_2S诱导2，4-二硝基苯醚在邻醛和HCl的辅助下硫解，因此具有高灵敏度和良好的选择性。相应的识别机理通过HRMS、1H核磁共振和对照实验得到了充分的证实。此外，该探针还成功地在不同的荧光通道中对细胞内的H_2S和HClO进行了分离和连续成像，表明该探针具有进一步研究H_2S和HClO在生命系统中的生物学功能的潜力。
Endogenous H2S and HClO play vital roles in regulating the redox status in vivo, and are involved in many pathophysiological events. Accordingly, accurate sensing and imaging of intracellular H2S and HClO have become greatly in demand. However, conventional double-response fluorescent probes for these two substances suffered from the notorious aggregation-caused quenching effect and required multiple excitations to achieve discrimination determination. In this study, we firstly constructed an AIE-active fluorescent probe with three potential reaction sites for individual and continuous detection of H2S and HClO using a single excitation wavelength. The multiple-site probe exhibited distinctive fluorescence behaviors to H2S and HClO with fast response (H2S: 12 min; HClO: 1.8 min), well-separated emission peaks (88 nm), high sensitivity and good selectivity due to H2S-induced thiolysis of 2,4-dinitrophenyl ether assisted by an adjacent aldehyde and HClOtriggered sulfur oxidation. The corresponding recognition mechanisms were fully confirmed by HRMS, 1H NMR and control experiments. Moreover, the probe was successfully utilized for separate and continuous imaging of intracellular H2S and HClO in different fluorescent channels, indicating its potential for further study the biological functions of H2S and HClO in living systems.