R-phycoerythrin proteins@ZIF-8 composite thin films for mercury ion detection
R-phycoerythrin proteins@ZIF-8 composite thin films for mercury ion detection
复制标题
R-藻红蛋白@ZIF-8复合薄膜用于汞离子检测
DOI：
10.1039/c9an00449a
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发表时间：
2019
期刊：
影响因子：
4.2
通讯作者：
Peng Xinsheng
中科院分区：
文献类型：
--
作者：
Wang Xiaobin;Fang Zhishan;Li Zhuoyi;Ying Wen;Chen Danke;He Haiping;Peng Xinsheng
汞是各种自然和人为过程释放的最普遍的有毒金属之一，对土壤和地下水造成严重污染。本工作采用固体限制转化法制备了R-藻红蛋白（R-PE）/ZIF-8复合薄膜，并将其作为荧光传感器用于汞离子的检测。包封到ZIF-8中的R-PE蛋白表现出包括绿色（518 nm）和红色（602，650 nm）荧光的双色发射，而纯R-PE的原始橙子发射（578 nm）被显著抑制。R-PE@ZIF-8在没有缓冲溶液的大pH范围内对汞的检测具有优异的选择性和灵敏度。在最佳条件下，R-PE@ZIF-8的荧光猝灭效率与汞离子的对数浓度在0.001 ~ 50 μM范围内呈良好的线性关系，检出限（LOD）为6.7 nM，远低于世界卫生组织给出的指导值。此外，R-PE@ZIF-8的多峰检测提高了Hg 2+浓度的检测精度。
Mercury, as one of the most prevalent toxic metals released by various natural and anthropogenic processes, causes severe pollution of soil and groundwater. In this work, R-phycoerythrin (R-PE) proteins encapsulated into ZIF-8 composite thin films were prepared via a solid-confinement conversion process and applied as fluorescent sensors for mercury ion detection. The R-PE proteins encapsulated into ZIF-8 exhibit dual color emissions including green (518 nm) and red (602, 650 nm) fluorescence, while the original orange emission (578 nm) of pure R-PE is significantly suppressed. R-PE@ZIF-8 presents excellent selectivity and sensitivity for mercury detection in a large pH range without buffer solution. Under the optimal conditions, there is a good linear relationship between the fluorescence quenching efficiencies of R-PE@ZIF-8 and logarithmic concentrations of mercury ions in the range of 0.001–50 μM with the detection limit (LOD) of 6.7 nM much lower than the guideline value given by the World Health Organization. Furthermore, multi-peak detection of R-PE@ZIF-8 improves the detection accuracy of Hg2+ concentration.