Protective effect of caffeic acid on cardiac markers and lipid peroxide metabolism in cardiotoxic rats: an in vivo and in vitro study
Protective effect of caffeic acid on cardiac markers and lipid peroxide metabolism in cardiotoxic rats: an in vivo and in vitro study
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DOI：
10.1016/j.metabol.2009.11.010
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发表时间：
2010-08-01
影响因子：
9.8
通讯作者：
Prince, P. Stanely Mainzen
中科院分区：
文献类型：
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作者：
Kumaran, K. Senthil;Prince, P. Stanely Mainzen
心肌梗塞影响着世界上大量人口。脂质过氧化物代谢在心肌梗死的病理学中起着重要作用。本研究旨在评估咖啡酸对异丙肾上腺素 (ISO) 诱导的心肌梗死雄性 Wistar 大鼠中脂质过氧化物、抗氧化剂、心脏标记酶和组织病理学结果的预防作用。通过皮下注射ISO(100mg/kg)，每隔24小时连续注射2天，诱导大鼠心肌梗塞。 ISO诱导的大鼠心脏中硫代巴比妥酸反应物质、脂质氢过氧化物、血浆尿酸和血清心脏标志酶的水平显着升高，而心脏超氧化物歧化酶、过氧化氢酶、谷胱甘肽过氧化物酶、谷胱甘肽还原酶、谷胱甘肽-S-转移酶的活性显着降低，血浆和心脏中还原型谷胱甘肽、维生素E和维生素C的水平显着降低。每日口服咖啡酸（15 mg/kg）预处理10天后，血清心脏标志酶、心脏脂质过氧化产物和血浆尿酸水平显着降低，抗氧化系统水平显着升高。心肌组织病理学也证实了咖啡酸对心肌梗塞大鼠的保护作用。总抗氧化活性的体外研究（2,2'-偶氮双-[3-乙基苯并噻唑啉-6-磺酸](+)测定）证实了咖啡酸的强大抗氧化作用。因此，本研究表明，咖啡酸由于其自由基清除和抗氧化作用，可通过维持脂质过氧化物代谢来改善 ISO 诱导的心肌梗死中的心脏损伤。含有咖啡酸的饮食可能对心肌梗塞有益。 (C) 2010 Elsevier Inc. 保留所有权利。
Myocardial infarction affects a large population in the world. Lipid peroxide metabolism plays an important role in the pathology of myocardial infarction. This study aims to evaluate the preventive effect of caffeic acid on lipid peroxides, antioxidants, cardiac marker enzymes, and histopathological findings in isoproterenol (ISO)-induced myocardial-infarcted male Wistar rats. Myocardial infarction was induced in rats by subcutaneous injection of ISO (100 mg/kg) at an interval of 24 hours for 2 days. The ISO-induced rats showed significant increase in the levels of thiobarbituric acid reactive substances, lipid hydroperoxides in the heart, plasma uric acid, and serum cardiac marker enzymes, and significant decrease in the activities of heart superoxide dismutase, catalase, glutathione peroxidase, glutathione reductase, glutathione-S-transferase, and the levels of reduced glutathione, vitamin E, and vitamin C in the plasma and heart. Oral pretreatment with caffeic acid (15 mg/kg) daily for 10 days showed significant decrease in the levels of serum cardiac marker enzymes, heart lipid peroxidation products and plasma uric acid and significant increase in the levels of antioxidant system. Histopathology of myocardium also confirmed the protective effect of caffeic acid in myocardial-infarcted rats. In vitro study on total antioxidant activity (2,2'-azinobis-[3-ethylbenzothiazoline-6-sulfonic acid](+) assay) confirmed the strong antioxidant action of caffeic acid. Thus, the present study revealed that caffeic acid ameliorates cardiac damage in ISO-induced myocardial infarction by maintaining lipid peroxide metabolism due to its free radical scavenging and antioxidant effects. A diet containing caffeic acid may be beneficial to myocardial infarction. (C) 2010 Elsevier Inc. All rights reserved.