Femtosecond Pulsed Laser as a Microscalpel for Microdissection and Isolation of Specific Sections from Biological Samples
Femtosecond Pulsed Laser as a Microscalpel for Microdissection and Isolation of Specific Sections from Biological Samples
复制标题
DOI：
10.1143/jjap.46.5859
复制
发表时间：
2007-09
影响因子：
1.5
通讯作者：
M. Sakakura;S. Kajiyama;Masafumi Tsutsumi;J. Si;E. Fukusaki;Y. Tamaru;Shinichi Akiyama;K. Miura;K. Hirao;M. Ueda
中科院分区：
文献类型：
--
作者：
M. Sakakura;S. Kajiyama;Masafumi Tsutsumi;J. Si;E. Fukusaki;Y. Tamaru;Shinichi Akiyama;K. Miura;K. Hirao;M. Ueda
飞秒激光是用于从生物样品中解剖和获得特定组织或细胞的候选工具。在本文中，我们报告的易燃（热敏）植物组织（Eurocumulmoides茎）和透明的斑马鱼胚胎放置在水中的解剖。在植物组织的激光切割过程中，我们观察到低重复率的辐射对于降低热损伤的风险至关重要。在激光切割斑马鱼胚胎的过程中，激光诱导的气泡在组织表面的形成干扰了激光诱导的切割。我们的结论是，“内部激光解剖方法”，其中的解剖是通过移动的激光光斑内的生物样品，是一种有效的方式来解剖样品，放置在水中。根据飞秒激光加工的物理特性，讨论了飞秒激光切割的优越性。
The femtosecond laser is a candidate tool for dissecting and obtaining specific tissues or cells from biological samples. In this paper, we report on the dissection of flammable (heat-sensitive) plant tissues (Eucommia ulmoides stem) and that of a transparent zebrafish embryo placed in water. During laser dissection in the plant tissues, we observed that a low repetition rate of the irradiations is crucial for reducing the risk of thermal damage. During laser dissection of the zebrafish embryo, the laser-induced bubble formation at the tissue surface disturbed the laser-induced dissection. We concluded that the “interior laser dissection method”, in which the dissection is performed by moving the laser spot inside a biological sample, is an efficient way to dissect a sample that is placed in water. In view of our findings, the advantages of fs laser dissection are discussed on the basis of the physical characteristics of fs laser machining.