The detection and characterisation of Dichelobacter nodosus from cases of ovine footrot in England and Wales
The detection and characterisation of Dichelobacter nodosus from cases of ovine footrot in England and Wales
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DOI：
10.1016/j.vetmic.2005.01.029
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发表时间：
2005-06-15
影响因子：
3.3
通讯作者：
Grogono-Thomas, R
中科院分区：
文献类型：
--
作者：
Moore, LJ;Wassink, GJ;Grogono-Thomas, R
在英国，由严格厌氧的结节性双螯菌引起的蹄病是绵羊跛行的最常见原因，但在诊断和控制方面存在问题。D. nodosus意味着需要复杂的培养基和数周的表征。提出了一种简化和增强D.因此，临床样品中的结节状突起是高度期望的。在控制方面，来自农业社区的轶事证据表明，基于澳大利亚分离的D。由于其被认为是无效的，因此在这个国家没有被广泛使用。因此，706株，收集在英格兰和威尔士的疫情，用于调查这些问题。采用16 S rRNA PCR方法检测D.在临床材料中的结节状的1天内采样; 15%的检测相比，培养和小于1%的假阴性。这代表了一个重大的进步，在快速诊断的蹄，并将是非常有价值的从业人员和诊断实验室。使用蛋白酶热稳定性和酶谱测定法测试细菌毒力，同时通过玻片凝集进行血清分型。所有分离株均表现出先前在澳大利亚记录的毒力模式，所有9个血清群的D。节瘤状体（A-I）。血清型H占优势。因此，没有证据表明存在新的D。与澳大利亚相比，这表明有必要进一步调查英国农民对使用商业疫苗的看法。(c)2005 Elsevier B. V.保留所有权利。
Footrot, caused by the strictly anaerobic bacterium Dichelobacter nodosus, is the most common cause of lameness in sheep in Great Britain but problems exist in association with its diagnosis and control. The fastidious nature of D. nodosus means that complex media and several weeks are required for characterisation. An alternative method to simplify and enhance the detection of D. nodosus in clinical samples is therefore highly desirable. In terms of control, anecdotal evidence from the farming community suggests that the commercially available vaccine, based on Australian isolates of D. nodosus, is not widely employed in this country due to its perceived inefficacy. Seven hundred and six isolates, collected from outbreaks in England and Wales, were therefore used to investigate these issues. A 16S rRNA PCR was adapted to detect D. nodosus in clinical material within 1 day of sampling; a 15% increase in detection compared with culture and less than 1% false negatives were achieved. This represents a major advance in the rapid diagnosis of footrot and will be of great value to practitioners and diagnostic laboratories. Bacterial virulence was tested using protease thermostability and zymogram assays, whilst serogrouping was performed by slide agglutination. All isolates demonstrated virulence patterns previously recorded in Australia and all nine serogroups of D. nodosus (A-I) were represented. Serogroup H was predominant. There was, therefore, no evidence for the presence of novel strains of D. nodosus compared with Australia suggesting the need for further investigation into farmers' views on the use of the commercial vaccine in Great Britain. (c) 2005 Elsevier B.V. All rights reserved.