Ca2+-Binding Proteins in Dogs with Heart Failure: Effects of Cardiac Contractility Modulation Electrical Signals
Ca2+-Binding Proteins in Dogs with Heart Failure: Effects of Cardiac Contractility Modulation Electrical Signals
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DOI：
10.1111/j.1752-8062.2009.00097.x
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发表时间：
2009-06-01
影响因子：
3.9
通讯作者：
Sabbah, Hani N.
中科院分区：
文献类型：
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作者：
Gupta, Ramesh C.;Mishra, Sudhish;Sabbah, Hani N.
背景：在患有心力衰竭（HF）的狗中，在绝对不应期向左心室（LV）肌肉传递心肌收缩力调节（CCM）电信号的长期治疗可改善左心室功能。本研究探讨了 CCM 治疗对心力衰竭犬钙 (Ca2+) 结合蛋白 (CBP) 表达的影响。 方法和结果：对七只接受 CCM 治疗的心力衰竭犬、七只未经治疗的心力衰竭犬和六只正常 (NL) 犬的左心室组织进行了研究。使用 RT-PCR 测量 S100A1、sorcin、早老素-1 (PS1)、PS2、富含组氨酸的 Ca2+ 结合蛋白 (HRC) 和管家基因 18S 核糖体 RNA (18S) 的 mRNA 表达。通过蛋白质印迹法测定 CBP 和钙螯合蛋白 (CSQ) 的蛋白质水平。研究组之间18S和CSQ的表达没有观察到差异。与NL相比，未治疗的HF犬中S100A1、sorcin和HRC的表达降低，而PS2的表达升高。 CCM 治疗使 S100A1、sorcin 和 PS2 的表达正常化，但不能使 HRC 的表达正常化。研究组之间PS1的表达没有变化。结论：CCM治疗恢复了S100A1、PS2和sorcin的LV表达。 CBP 正常化可能部分有助于 CCM 治疗后心力衰竭患者左心室功能的改善。
Background: In dogs with heart failure (HF), chronic therapy with cardiac contractility modulation (CCM) electrical signals delivered to left ventricular (LV) muscle during the absolute refractory period improves LV function. This study examined the effects of CCM therapy on the expression of calcium (Ca2+)-binding proteins (CBPs) in dogs with HF.Methods and Results: Studies were performed in LV tissue from seven CCM-treated HF dogs, seven untreated HF dogs, and six normal (NL) dogs. mRNA expression of S100A1, sorcin, presenillin-1 (PS1), PS2, histidine-rich Ca2+-binding protein (HRC), and 18S ribosomal RNA (18S), a housekeeping gene, was measured using RT-PCR. Protein levels of CBPs and calsequestrin (CSQ) were determined by Western blotting. No difference was observed in the expression of 18S and CSQ among study groups. Compared with NL, the expression of S100A1, sorcin, and HRC was decreased, whereas the expression of PS2 was increased in untreated HF dogs. CCM therapy normalized the expression of S100A1, sorcin, and PS2 but not of HRC. No change was seen in the expression of PS1 among study groups.Conclusion: CCM therapy restores LV expression of S100A1, PS2, and sorcin. Normalization of CBPs may partly contribute to improved LV function in HF following CCM therapy.