Comparison of Growth Factor and Platelet Concentration From Commercial Platelet-Rich Plasma Separation Systems
Comparison of Growth Factor and Platelet Concentration From Commercial Platelet-Rich Plasma Separation Systems
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DOI：
10.1177/0363546510387517
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发表时间：
2011-02-01
影响因子：
4.8
通讯作者：
Dragoo, Jason L.
中科院分区：
文献类型：
--
作者：
Castillo, Tiffany N.;Pouliot, Michael A.;Dragoo, Jason L.
背景资料：临床研究声称，富血小板血浆（PRP）缩短了恢复时间，因为它的高浓度的生长因子，可以增强组织修复过程。大多数这些研究获得PRP使用不同的分离系统，很少分析的PRP作为treatment.Purpose的内容：本研究的特点是由3个市售PRP分离systems.Study设计：对照实验室研究的单供体PRP的组成。研究方法：5名健康人捐献了100 mL血液，使用3种PRP浓缩系统（MTF Cascade、Arteriocyte Magellan、Biomet GPS III）对血液进行处理以产生PRP。血小板、白色血细胞（WBC）、红细胞和纤维蛋白原浓度通过临床实验室中的自动化系统进行分析，而ELISA测定血小板衍生生长因子α β和β β的浓度（PDGF-α β，PDGF-β β），转化生长因子β 1（TGF-β 1）和血管内皮生长因子（VEGF）。PRP分离系统之间的平均PRP血小板、红细胞、活性TGF-β 1或纤维蛋白原浓度无显著差异。血小板捕获效率有显著差异。Cascade的血小板捕获效率最高，与Magellan相当，但显著高于GPS III。在所有系统中，WBC、PDGF-α β、PDGF-β β和VEGF的浓度存在显著差异。Cascade系统浓缩了白细胞贫乏的PRP，与来自GPS III和Magellan system.Conclusion的白细胞丰富的PRP相比：GPS III和Magellan浓缩了白细胞丰富的PRP，这导致白细胞、PDGF-α β、PDGF-β β和VEGF的浓度增加，与来自Cascade的白细胞贫乏的PRP相比。总体而言，系统间血小板浓度、红细胞、活性TGF-β 1或纤维蛋白原水平无显著差异。
Background: Clinical studies claim that platelet-rich plasma (PRP) shortens recovery times because of its high concentration of growth factors that may enhance the tissue repair process. Most of these studies obtained PRP using different separation systems, and few analyzed the content of the PRP used as treatment.Purpose: This study characterized the composition of single-donor PRP produced by 3 commercially available PRP separation systems.Study Design: Controlled laboratory study. Methods: Five healthy humans donated 100 mL of blood, which was processed to produce PRP using 3 PRP concentration systems (MTF Cascade, Arteriocyte Magellan, Biomet GPS III). Platelet, white blood cell (WBC), red blood cell, and fibrinogen concentrations were analyzed by automated systems in a clinical laboratory, whereas ELISA determined the concentrations of platelet-derived growth factor alpha beta and beta beta (PDGF-alpha beta, PDGF-beta beta), transforming growth factor beta 1 (TGF-beta 1), and vascular endothelial growth factor (VEGF).Results: There was no significant difference in mean PRP platelet, red blood cell, active TGF-beta 1, or fibrinogen concentrations among PRP separation systems. There was a significant difference in platelet capture efficiency. The highest platelet capture efficiency was obtained with Cascade, which was comparable with Magellan but significantly higher than GPS III. There was a significant difference among all systems in the concentrations of WBC, PDGF-alpha beta, PDGF-beta beta, and VEGF. The Cascade system concentrated leukocyte-poor PRP, compared with leukocyte-rich PRP from the GPS III and Magellan systems.Conclusion: The GPS III and Magellan concentrate leukocyte-rich PRP, which results in increased concentrations of WBCs, PDGF-alpha beta, PDGF-beta beta, and VEGF as compared with the leukocyte-poor PRP from Cascade. Overall, there was no significant difference among systems in the platelet concentration, red blood cell, active TGF-beta 1, or fibrinogen levels.