DII REVEALS A PRENATAL ARRIVAL OF EFFERENTS AT THE DIFFERENTIATING OTOCYST OF MICE
DII REVEALS A PRENATAL ARRIVAL OF EFFERENTS AT THE DIFFERENTIATING OTOCYST OF MICE
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DOI：
10.1016/0378-5955(93)90200-k
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发表时间：
1993-02-01
期刊：
影响因子：
2.8
通讯作者：
NICHOLS, DH
中科院分区：
文献类型：
--
作者：
FRITZSCH, B;NICHOLS, DH
我们已经重新调查的时间传出纤维在发展中的小鼠耳囊采用扩散的亲脂性染料二碘在固定的组织。在几乎所有以前的报告相反，我们的数据表明，产前传出纤维的到来。在胚胎艾德10 1/2日龄时，有少数传出纤维进入第8神经根。逆行标记的传出细胞体在这个阶段coextensive与那些面运动核，但开始分离的艾德12。相反，逆行标记的面运动神经元，标记的传出神经元双侧分布在后脑，有几个投影到两个耳囊早在艾德12。从大脑的顺行标记显示，在艾德11前庭神经节传出纤维。在艾德12时开始侵袭未来的前庭感觉上皮。传出纤维的生长锥也达到了未来的耳蜗感觉上皮，但在这个阶段，只有少数丝状伪足实现入侵。早期到达传出神经在未来的感觉上皮细胞证明这里可能会允许一个尚未探索的相互作用的传出纤维与增殖和/或分化的毛细胞。
We have reinvestigated the time of arrival of efferent fibers at the developing otocyst of mice employing diffusion of the lipophilic dye DiI in fixed tissue. In contrast to almost all previous reports, our data indicate a prenatal arrival of efferent fibers. A few efferent fibers were found to enter the eighth nerve root at embryonic day (ED) 10 1/2. Retrogradely labelled efferent cell bodies were at this stage coextensive with those of the facial motor nucleus, but started to segregate by ED 12. In contrast to retrogradely labelled facial motor neurons, labelled efferent neurons were bilaterally distributed in the hindbrain with a few projecting to both otocysts as early as ED 12. Anterograde labelling from the brain showed efferent fibers in the vestibular ganglion by ED 11. Invasion of the future vestibular sensory epithelia started by ED 12. Growth cones of efferent fibers had also reached the future cochlear sensory epithelium but invasion was only achieved by a few filopodia at this stage. The early arrival of efferents at the future sensory epithelia demonstrated here may allow an as yet unexplored interaction of efferent fibers with the proliferating and/or differentiating hair cells.