Sequential DNA methylation of the Nanog and Oct-4 upstream regions in human NT2 cells during neuronal differentiation
Sequential DNA methylation of the Nanog and Oct-4 upstream regions in human NT2 cells during neuronal differentiation
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DOI：
10.1074/jbc.c400479200
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发表时间：
2005-02-25
影响因子：
4.8
通讯作者：
Walker, PR
中科院分区：
文献类型：
--
作者：
Deb-Rinker, P;Ly, D;Walker, PR
分化为神经元和星形胶质细胞的人NT 2细胞最初表达Nanog和Oct-4（POU 51 F1），然后永久下调。我们研究了这些基因的表达与其5-侧翼区甲基化状态之间的关系。基因表达和DNA甲基化分别用定量聚合酶链反应和亚硫酸氢盐基因组测序法测定。视黄酸诱导的NT 2细胞分化为神经元伴随着两个基因表达的顺序降低，并通过其上游区域的顺序表观遗传修饰来实现。这是第一份证明人类细胞系中Nanog和Oct-4启动子区域DNA甲基化变化的报告。
Human NT2 cells, which differentiate into neurons and astrocytes, initially express and then permanently down-regulate Nanog and Oct-4 (POU51F1). We investigated the relationship between the expression of these genes and the methylation state of their 5-flanking regions. Gene expression and DNA methylation were assayed with quantitative polymerase chain reaction and bisulfite genomic sequencing, respectively. Retinoic acid-induced differentiation of NT2 cells to neurons is accompanied by a sequential decrease in the expression of both genes, paralleled by sequential epigenetic modification of their upstream regions. This is the first report demonstrating changes in DNA methylation in the promoter regions of Nanog and Oct-4 in a human cell line.