Natural variation of nitrate uptake and nitrogen use efficiency in Arabidopsis thaliana cultivated with limiting and ample nitrogen supply
Natural variation of nitrate uptake and nitrogen use efficiency in Arabidopsis thaliana cultivated with limiting and ample nitrogen supply
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DOI：
10.1093/jxb/erq059
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发表时间：
2010-05-01
影响因子：
6.9
通讯作者：
Masclaux-Daubresse, Celine
中科院分区：
文献类型：
--
作者：
Chardon, Fabien;Barthelemy, Julien;Masclaux-Daubresse, Celine
以18份拟南芥为材料，在低（N-）和高（N+）供氮条件下生长。通过饲喂富N-15营养液监测植株对氮的吸收，监测并计算生物量[鲜物质（FM）和干物质（DM）]、N浓度（N%）和N-15含量，以确定氮素利用效率（NUE）和氮吸收效率（NupE）。NUE为生物量与N浓度之比（DM/N%）， NupE为植物体内N-15的浓度[mu g(g(-1) DM)]。分析加入性状以检测共同和个体基因型特征。高氮输入条件下氮素利用效率的遗传变异主要通过氮素吸收的变异来解释。尽管在N+和N-条件下植物对氮的吸收和代谢方式不同，但氮素利用效率在这两种条件下是相似的，表明氮素利用效率完全由遗传决定。等级分类表明，在N-和N+条件下产生的生理分类相似。同时检测到浪费型和高效型基因型。发现了3种极端基因型：Col-0、Bur-0和Tsu-0。Bur-0和su-0表现出较高的氮肥利用率和较大的生物量。Col-0则相反：低氮肥利用率和低生物量。bur0对高氮的耐受性较差。目前的数据将有助于选择拟南芥作为重组自交系群体的亲本，以适合与NUE， NupE和N储存容量相关的定量性状位点的定位。
Eighteen accessions of Arabidopsis thaliana were grown with low (N-) and high (N+) nitrogen supply. N uptake was monitored by feeding plants with N-15-enriched nutritive solution over 24 h. Biomass [fresh matter (FM) and dry matter (DM)], N concentration (N%), and N-15 content were monitored and computed to determine the nitrogen use efficiency (NUE) and nitrogen uptake efficiency (NupE). NUE has been estimated as the ratio between biomass and N concentration (DM/N%) and NupE as the concentration of N-15 in plants [mu g (g(-1) DM)]. Accession traits were analysed to detect common and individual genotype features. The genetic variation in NUE at high N input was mainly explained by variation in N uptake. Even though plants managed N uptake and N metabolism differently under N+ and N-, NUE was similar in these two conditions, showing that NUE was exclusively genetically determined. Hierarchical classification revealed that the physiological classes arising were similar under N- and N+. Both wasteful and efficient genotypes were detected. Three extreme genotypes, Col-0, Bur-0, and Tsu-0, were noted. Bur-0 and Tsu-0 exhibited high NUE and large biomass. Col-0 showed the reverse: low NUE and low biomass. Bur-0 appeared poorly tolerant of a high N supply. The present data will facilitate the choice of Arabidopsis accessions as parents of recombinant inbred line populations suitable for the mapping of quantitiative trait loci related to NUE, NupE, and N storage capacity.