An Improved Radioenzymatic Assay for Histamine in Human Plasma, Whole Blood, Urine, and Gastric Juice
An Improved Radioenzymatic Assay for Histamine in Human Plasma, Whole Blood, Urine, and Gastric Juice
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改进的放射酶法测定人血浆、全血、尿液和胃液中的组胺
DOI：
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发表时间：
1979
影响因子：
2.2
通讯作者：
A. Westwood
中科院分区：
文献类型：
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作者：
C. Bruce;W. Taylor;A. Westwood
描述了一种适用于测定人体血液、尿液、血浆和胃液中组胺的放射性酶法。它与早期方法的不同之处在于使用来自猪脑的组胺甲基转移酶制剂、高活性氚化S-腺苷甲硫氨酸以及热沉淀步骤以减少先前提到的血浆成分的干扰。该方法比需要溶剂萃取和浓缩组胺的方法更简单，回收率在 80-120% 范围内，因此无需内部标准化。该方法灵敏、精确，血液、尿液和血浆的变异系数分别为5%、6%和13%。正常人血浆组胺的平均值±标准差为 5 ± 4 nmol/l，全血为 559 ± 193 nmol/l，尿液为 229 ± 128 nmol/24h。
A radioenzymatic method suitable for the assay of histamine in human blood, urine, plasma, and gastric juice is described. It differs from earlier methods by use of a histamine methyltransferase preparation from pig brain, of high activity tritiated S-adenosylmethionine, and of a heat precipitation step to reduce the previously noted interference from plasma constituents. The method is simpler than those requiring solvent extraction and concentration of histamine, gives recoveries in the range 80–120%, and so eliminates the need for internal standardisation. The method is sensitive and precise with coefficients of variation for blood, urine, and plasma of 5%, 6%, and 13% respectively. The mean ± standard deviation for normal human plasma histamine is 5 ± 4 nmol/l, for whole blood 559 ± 193 nmol/l, and for urine 229 ± 128 nmol/24h.