Mechanical stretch promotes proliferation of rat bone marrow mesenchymal stem cells
Mechanical stretch promotes proliferation of rat bone marrow mesenchymal stem cells
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DOI：
10.1016/j.colsurfb.2007.04.001
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发表时间：
2007-08-01
影响因子：
5.8
通讯作者：
Qin, Jian
中科院分区：
文献类型：
--
作者：
Song, Guanbin;Ju, Yang;Qin, Jian
人体内的组织和细胞持续暴露在复杂的机械环境中。机械刺激对活细胞的形态、发育和功能状态至关重要，施加于细胞的机械刺激的方式被认为对诱导的细胞反应和功能极其重要。在这项研究中，我们调查是否机械拉伸调节和促进大鼠骨髓间充质干细胞（rMSCs）在体外增殖。分离纯化大鼠骨髓间充质干细胞，经等轴拉伸处理后，采用MTT比色法和RT-PCR检测c-fos基因表达，观察细胞增殖情况。结果表明：1 Hz牵张15-60 min和2-8%应变后，rMSCs的OD值呈时间依赖性和幅度依赖性增加。牵张处理（1Hz，8%应变，60 min）的rMSCs中c-fos基因的表达显著高于未受牵张处理的对照细胞。这些结果表明，机械牵张在调节细胞生长和增殖方面发挥着重要作用，适当的机械牵张处理可以促进rMSC的增殖能力。(C)2007 Elsevier B. V.保留所有权利。
Tissues and cells in the body are continuously exposed to a complex mechanical environment. Mechanical stimulations are critical to morphological, developmental and functional states of living cells, and the fashion of the mechanical stimulation applied to the cells is supposed to be extremely important for the induced cell response and function. In this study, we investigated whether mechanical stretch regulates and promotes proliferation of rat bone marrow mesenchymal stem cells (rMSCs) in vitro. rMSCs from rat bone marrow were isolated, purified and subjected to a cyclic equiaxial stretch treatment, and then MTT assay was adopted and expression of c-fos gene was measured by RT-PCR to access cell proliferation. The results demonstrated that OD values of rMSCs increased in a time-dependent and magnitude-dependent manner after exposure to I Hz stretch within 15-60 min and 2-8% strain. Expression of c-fos gene in rMSCs subjected to stretch treatment (1 Hz, 8% strain and 60 min) is significantly higher than that of unstimulated control cells. These results suggest that mechanical stretch plays an important role in regulating the cell growth and proliferation, and an appropriate mechanical stretch treatment could promote proliferating capacity of rMSCs. (C) 2007 Elsevier B.V. All rights reserved.