Burkholderia sprentiae sp nov., isolated from Lebeckia ambigua root nodules
Burkholderia sprentiae sp nov., isolated from Lebeckia ambigua root nodules
复制标题
DOI：
10.1099/ijs.0.048777-0
复制
发表时间：
2013-11-01
影响因子：
2.8
通讯作者：
Vandamme, Peter
中科院分区：
文献类型：
--
作者：
De Meyer, Sofie E.;Cnockaert, Margo;Vandamme, Peter
从甜菜根瘤中分离到7株革兰氏阴性杆状细菌，并在该寄主上进行了鉴定。根据16S rRNA基因系统发育分析，它们属于伯克霍尔德氏菌属，其中代表性菌株WSM5005(T)与伯克霍尔德氏菌的亲缘关系最近(序列相似性为98.08%)。此外，这些菌株在基于看家基因gyrB和recA的系统发育树上形成了一个不同的组。化学分类数据，包括脂肪酸图谱和呼吸对苯二酚的分析，支持将菌株归入伯克霍尔德氏菌属。DNA-DNA杂交和生理生化测试的结果允许我们的菌株与伯克霍尔德氏菌属中最接近的物种进行基因和表型区分，并具有有效的公布名称。因此，这些菌株代表了一个新的物种，其名称为Burkholderia sp.sp.11月提出了模式菌株WSM5005(T)=1MG 27175(T)=Hambi3357(T)。
Seven Gram-stain-negative, rod-shaped bacteria were isolated from Lebeckia ambigua root nodules and authenticated on this host. Based on the 16S rRNA gene phylogeny, they were shown to belong to the genus Burkholderia, with the representative strain WSM5005(T) being most closely related to Burkholderia tuberum (98.08% sequence similarity). Additionally, these strains formed a distinct group in phylogenetic trees based on the housekeeping genes gyrB and recA. Chemotaxonomic data including fatty acid profiles and analysis of respiratory quinones supported the assignment of the strains to the genus Burkholderia. Results of DNA-DNA hybridizations, and physiological and biochemical tests allowed genotypic and phenotypic differentiation of our strains from the closest species of the genus Burkholderia with a validly published name. Therefore, these strains represent a novel species for which the name Burkholderia sprentiae sp. nov. (type strain WSM5005(T)=LMG 27175(T)=HAMBI 3357(T)) is proposed.