Interaction of divalent cations with germ cell specific sulfogalactosylglycerolipid and the effects on lipid chain dynamics.
Interaction of divalent cations with germ cell specific sulfogalactosylglycerolipid and the effects on lipid chain dynamics.
复制标题
二价阳离子与生殖细胞特异性磺基半乳糖甘油脂的相互作用及其对脂质链动力学的影响。
DOI：
10.1021/bi00249a011
复制
发表时间：
1994
期刊：
影响因子：
2.9
通讯作者：
N. Tanphaichitr
中科院分区：
文献类型：
--
作者：
S. Tupper;P. Wong;M. Kates;N. Tanphaichitr
磺基半乳糖甘油脂(SGG)是一种广泛存在于哺乳动物雄性生殖细胞质膜中的磺基糖脂。尽管其确切的细胞功能尚不清楚(S)，但推测SGG可能在阳离子转运中发挥作用，这可能在精卵相互作用中起重要作用。鉴于Ca~(2+)在许多受精相关事件中的重要作用，本研究的目的是确定Ca~(2+)与SGG中带负电荷的硫酸盐基团的相互作用是否会导致SGG脂链分子动力学的变化，并将这些脂质动力学与Na~+、Mg~(2+)或Sr~(2+)与SGG相互作用的结果进行比较。本研究采用压力调谐傅里叶变换红外光谱技术。结果表明，三种二价阳离子均与水合SGG的硫酸盐部分发生静电相互作用，但强度不同。结果发现，水合SGG-Na+多层膜的碳氢链相互交错，从而增加了碳氢链末端CH3基团的无序性。三种二价阳离子的存在都消除了这种交错状态。据推测，这是通过每个二价阳离子与邻近脂分子的硫酸盐基团的交联作用来实现的。此外，二价阳离子相互作用增加了SGG的脂链动力学，其中镁离子引起的链紊乱最大，其次是钙离子，然后是锶离子。链无序的增加会增加双层的流动性。这种现象可能与受精相关事件中观察到的精子质膜变化有关。
Sulfogalactosylglycerolipid (SGG) is a sulfoglycolipid found ubiquitously in the plasma membrane of mammalian male germ cells. Although its exact cellular function(s) is unknown, it is speculated that SGG may play a role in cation transport, which may be important in sperm-egg interaction. Given the significant role of Ca2+ in many fertilization-related events, the purpose of this study was to determine whether Ca2+ interaction with the negatively charged sulfate group of SGG results in changes to the SGG lipid chain molecular dynamics and to compare these lipid dynamics with those resulting from Na+, Mg2+, or Sr2+ interaction with SGG. Pressure-tuning Fourier transform infrared spectroscopy was used in this study. The results obtained showed that all three divalent cations interacted electrostatically with the sulfate moiety of hydrated SGG, although with varying degrees of strength. It was found that the hydrocarbon chains of hydrated SGG-Na+ multilamellar bilayers were interdigitated, thus increasing disorderedness of the terminal CH3 group of the hydrocarbon chains. The presence of each of the three divalent cations abolished this interdigitation state. Presumably, this is through the cross-linking interaction of each divalent cation with the sulfate groups of neighboring lipid molecules. Moreover, divalent cation interaction was found to increase the lipid chain dynamics of SGG, with Mg2+ inducing the greatest chain disorder followed by Ca2+ and then Sr2+. An increase in chain disorder would increase the bilayer fluidity. Such a phenomenon may prove relevant to the changes observed in the sperm plasma membrane during fertilization-related events.
登录
查看更多内容
影响因子：
2.7
作者：
FLORMAN, HM;CORRON, ME;BABCOCK, DF
通讯作者：
BABCOCK, DF
DOI：
10.1139/o85-134
发表时间：
1985
期刊：
Canadian journal of biochemistry and cell biology = Revue canadienne de biochimie et biologie cellulaire
影响因子：
--
作者：
Lingwood,CA
通讯作者：
Lingwood,CA
哺乳动物组织中硫脂的代谢。
DOI：
10.1016/b978-0-12-024918-3.50010-1
发表时间：
1981
期刊：
Advances in lipid research
影响因子：
--
作者：
Farooqui,AA
通讯作者：
Farooqui,AA
磺基半乳糖酰基烷基甘油（SGG）及其结合蛋白在精子发生和精子成熟过程中的共定位。
DOI：
10.1139/o86-131
发表时间：
1986
期刊：
Biochemistry and cell biology = Biochimie et biologie cellulaire
影响因子：
--
作者：
Lingwood,CA
通讯作者：
Lingwood,CA
牛精子精磷脂的高分辨率质子核磁共振表征。
影响因子：
6.5
作者：
Alvarez,JG;Storey,BT;Hemling,ML;Grob,RL
通讯作者：
Grob,RL