Bacterial community dynamics in a full-scale municipal wastewater treatment plant employing conventional activated sludge process
Bacterial community dynamics in a full-scale municipal wastewater treatment plant employing conventional activated sludge process
复制标题
DOI：
10.1016/j.jbiosc.2013.12.008
复制
发表时间：
2014-07-01
影响因子：
2.8
通讯作者：
Ike, Michihiko
中科院分区：
文献类型：
--
作者：
Hashimoto, Kurumi;Matsuda, Masami;Ike, Michihiko
为了阐明大型污水处理厂 (WWTP) 中的细菌群落动态以及进水、出水和污泥中细菌群落之间的相关性，对采用传统活性污泥工艺的大型污水处理厂中细菌群落的结构和代谢能力进行了为期 10 个月的研究。通过针对真细菌16S rRNA基因的末端限制性片段长度多态性分析细菌群落结构，同时采用Biolog测定法评估活性污泥的代谢能力。进水细菌群落结构总体稳定。相比之下，出水中的细菌群落结构在某些情况下与进水相似，而在其他情况下却是独特的，与进水和污泥中的细菌群落结构有很大差异。这些结果表明，废水细菌群落的时间变化可能有助于预测废水处理性能和活性污泥的沉降性。污泥中细菌群落结构相对稳定，很少受进水种群的影响。 Biolog 分析还表明，由于功能冗余，活性污泥随着时间的推移保持了非常相似的有机化合物代谢潜力，其中少数群体发挥了重要作用。 (C) 2013 年，日本生物技术协会。版权所有。
To elucidate the bacterial community dynamics in a full-scale wastewater treatment plant (WWTP) and the relatedness among bacterial communities in the influent, effluent and sludge, the structure and metabolic ability of the bacterial community throughout a full-scale WWTP employing a conventional activated sludge process was investigated during a period of 10 months. The bacterial community structure was analyzed by terminal-restriction fragment length polymorphism targeting eubacterial 16S rRNA genes, while a Biolog assay was applied to assess the metabolic ability of the activated sludge. Influent bacterial community structure was generally stable. In contrast, the bacterial community structure in the effluent was similar to that in the influent in some cases, while in other cases it was unique and differed greatly from that in the influent and sludge. These results suggest that temporal variations of the effluent bacterial community may be useful to predict the wastewater treatment performance and settleability of activated sludge. The bacterial community structure in the sludge was relatively stable and was rarely impacted by the influent populations. Biolog assay also revealed that activated sludge maintained a remarkably similar metabolic potential of organic compounds over time due to functional redundancy, in which the minor populations played a significant role. (C) 2013, The Society for Biotechnology, Japan. All rights reserved.