Sequential transition of the injury phenotype, temperature-dependent survival and transcriptional response in Listeria monocytogenes following lethal H2O2 exposure.
Sequential transition of the injury phenotype, temperature-dependent survival and transcriptional response in Listeria monocytogenes following lethal H2O2 exposure.
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DOI：
10.1016/j.ijfoodmicro.2017.08.001
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发表时间：
2017-10
影响因子：
5.4
通讯作者：
Y. Ochiai;F. Yamada;Y. Yoshikawa;M. Mochizuki;T. Takano;R. Hondo;F. Ueda
中科院分区：
文献类型：
--
作者：
Y. Ochiai;F. Yamada;Y. Yoshikawa;M. Mochizuki;T. Takano;R. Hondo;F. Ueda
食源性病原体单核细胞增生李斯特菌持续存在于食品加工环境中，其中该细菌暴露于各种应激因素，包括氧化应激。本研究旨在阐明L.单核细胞生成H2O2暴露和H2O2处理的细菌菌落形成的表型变化。存活曲线表明对H_2O_2的抗性增加。单核细胞生成的温度下降，在应力暴露程序。在37 °C下观察到在响应H2O2中具有关键作用的基因（包括sigBandkat）的转录诱导，但在20 °C下未观察到，而其他应激反应基因在两种温度下均被诱导。H2O2胁迫后，L.单核细胞增多症产生小集落表型，并且殖民地大小以应激暴露持续时间依赖的方式减小。复苏的细胞没有能力形成集落在丙酮酸钠的情况下也found.Our的研究结果表明，在损伤表型从小集落表型复苏的细胞在暴露于H2O2的过程中发生的顺序过渡的可能性。在20 °C下比37 °C下更高的H2O2抗性表明进一步研究了在较低温度下（包括冷藏温度）对H2O2暴露的反应，这可能有助于阐明食品加工环境中细菌在延长时间内的存活情况。
The food-borne pathogenListeria monocytogenesis present persistently in food processing environments, where this bacterium is exposed to various stress factors, including oxidative stress. This study aimed to elucidate the temperature-dependent response ofL. monocytogenesto H2O2exposure and the phenotypic changes in colony formation by H2O2-treated bacteria. Survival curves indicated an increase in the resistance to H2O2inL. monocytogenesas the temperature decreased during the stress exposure procedure. Transcriptional induction of genes with key roles in response to H2O2, includingsigBandkat, was observed at 37 °C, but not at 20 °C, whereas other stress response genes were induced at both temperatures. Following H2O2exposure,L. monocytogenesproduced small colony phenotypes and the colony size decreased in a stress exposure duration-dependent manner. Resuscitated cells with no ability to form colonies in the absence of sodium pyruvate were also found. Our findings show the possibility that a sequential transition in the injury phenotype from small colony phenotype to resuscitated cells occurred during the course of exposure to H2O2. The higher H2O2resistance at 20 °C than 37 °C suggests further investigation of the response to H2O2exposure under the lower temperatures, including refrigeration temperature, which may contribute to elucidation of bacterial survival over extended time periods in food-processing environments.