Mapping of quantitative trait loci controlling low-temperature germinability in rice (Oryza sativa L.)
Mapping of quantitative trait loci controlling low-temperature germinability in rice (Oryza sativa L.)
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DOI：
10.1007/s00122-003-1509-4
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发表时间：
2004-03-01
影响因子：
5.4
通讯作者：
Yano, M
中科院分区：
文献类型：
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作者：
Fujino, K;Sekiguchi, H;Yano, M
在温带地区和热带高海拔地区，低温发芽是直播法建立稳定林分的主要决定因素之一。以温带粳稻品种Italica利沃诺和Hayamasari杂交获得的122个回交自交系为材料，分析了控制水稻低温发芽力的数量性状位点（QTL）。测定15 ℃下的发芽率以代表低温发芽，并用于QTL分析。在15 ℃下7天，意大利利沃诺和Hayamasari的发芽率分别为98.7%和26.8%，BILs的发芽率为0 ~ 83.3%。利用限制性片段长度多态性（RFLP）和简单重复序列（SSR）标记，构建了一张连锁图谱，该图谱覆盖了水稻基因组的90%左右。检测到3个与低温发芽相关的QTL。位于第3染色体上的qLTG-3-1是影响低温发芽力的最有效QTL，其贡献率为35.0%。另外还检测到2个QTL，位于第3染色体上的qLTG-3-2和位于第4染色体上的qLTG-4，分别占总表型变异的17.4%和5.5%。在所有检测到的QTL中，Italica利沃诺等位基因提高了低温发芽率。
Low-temperature germination is one of the major determinants for stable stand establishment in the direct seeding method in temperate regions, and at high altitudes of tropical regions. Quantitative trait loci (QTLs) controlling low-temperature germinability in rice were identified using 122 backcross inbred lines (BILs) derived from a cross between temperate japonica varieties, Italica Livorno and Hayamasari. The germination rate at 15degreesC was measured to represent low-temperature germination and used for QTL analysis. The germination rate at 15degreesC for 7 days of Italica Livorno and Hayamasari was 98.7 and 26.8%, respectively, and that of BILs ranged from 0 to 83.3%. Using restriction fragment length polymorphism (RFLP) and simple sequence repeat (SSR) markers, we constructed a linkage map which corresponded to about 90% of the rice genome. Three putative QTLs associated with low-temperature germination were detected. The most effective QTL, qLTG-3-1 on chromosome 3, accounted for 35.0% of the total phenotypic variation for low-temperature germinability. Two additional QTLs, qLTG-3-2 on chromosome 3 and qLTG-4 on chromosome 4, were detected and accounted for 17.4 and 5.5% of the total phenotypic variation, respectively. The Italica Livorno alleles in all detected QTLs increased the low-temperature germination rate.