Distribution of myeloid dendritic cells and plasmacytoid dendritic cells in the synovial tissues of rheumatoid arthritis.
Distribution of myeloid dendritic cells and plasmacytoid dendritic cells in the synovial tissues of rheumatoid arthritis.
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发表时间：
2008-10
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通讯作者：
Y. Takakubo;M. Takagi;K. Maeda;Y. Tamaki;A. Sasaki;T. Asano;S. Fukushima;Y. Kiyoshige;H. Orui;T. Ogino;M. Yamakawa
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作者：
Y. Takakubo;M. Takagi;K. Maeda;Y. Tamaki;A. Sasaki;T. Asano;S. Fukushima;Y. Kiyoshige;H. Orui;T. Ogino;M. Yamakawa
目的 检查类风湿性关节炎 (RA) 和骨关节炎 (OA) 患者滑液组织和滑液 (SF) 中树突状细胞 (DC) 和吲哚胺 2,3-双加氧酶 (IDO) 表达细胞的精确组织分布。方法 对 30 名 RA 患者和 7 名 OA 患者的滑膜组织进行 DC 标记物的免疫组织化学染色。根据病理生物学分期和组织病理学分级系统，检查区域分为 5 类。使用正磁分选系统和负磁分选系统分别从 SF 样本（7 名 RA 患者和 4 名 OA 患者）和滑膜组织（3 名 RA、4 名 OA）中分离出髓样 DC (mDC) 和浆细胞样 DC (pDC)。结果mDC主要表现为淋巴聚集。 pDC 散布在静脉周围浸润区域，RA 中则有大大小小的淋巴聚集。与 OA 滑膜组织相比，RA SF 组织中的 mDC/pDC 比率显着增加，分级更高（p<0.05）。通过对 RA 滑膜组织进行连续切片和染色，检测 pDC 中的 IDO 免疫反应性。结论 我们的结果表明成熟的 mDC 在 RA 炎症过程中发挥着核心作用。尽管 pDC 少于 mDC，但 IDO 阳性 pDC 的存在表明 RA 滑膜组织中可能存在耐受机制。然而，由于 RA 中明显的炎症（其中 mDC 占主导地位），这种影响可能不大。
OBJECTIVE To examine the precise tissue distribution of dendritic cells (DC) and indoleamine 2,3-dioxygenase (IDO)-expressing cells in synovial tissue and synovial fluid (SF) from patients with rheumatoid arthritis (RA) and osteoarthritis (OA). METHODS Synovial tissues from 30 patients with RA and 7 with OA were immunohistochemically stained for DC markers. The examined areas were classified into 5 categories based on pathobiological staging and histopathological grading systems. Myeloid DC (mDC) and plasmacytoid DC (pDC) were isolated using positive and negative magnetic sorting systems, respectively, from SF samples (7 patients with RA and 4 with OA) and synovial tissues (3 RA, 4 OA). RESULTS mDC were mainly observed in lymphoid aggregations. pDC were scattered around perivenular infiltration areas, and small and large lymphoid aggregations in RA. The mDC/pDC ratio increased significantly, with higher grading in RA SF tissues compared to OA synovial tissues (p<0.05). IDO-immunoreactivity was detected in pDC by serial sectioning and staining of RA synovial tissues. CONCLUSION Our results indicate that mature mDC play a central role in the RA inflammatory process. Although there were fewer pDC than mDC, the presence of IDO-positive pDC suggests a possible tolerance mechanism in RA synovial tissues. However, it is probably modest due to the marked inflammation in RA, in which mDC are dominant.