Biofilm Formation in the 96-Well Microtiter Plate
Biofilm Formation in the 96-Well Microtiter Plate
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DOI：
10.1007/978-1-4939-0473-0_48
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发表时间：
2014-01-01
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影响因子：
--
通讯作者：
Anderson, Gregory G.
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文献类型：
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作者：
Coffey, Barbara M.;Anderson, Gregory G.
用于研究生物膜形成的微量滴定板（也称为96孔板）测定是允许观察细菌粘附到非生物表面的方法。在该测定中，将细菌在乙烯基“U”底或其他类型的96孔微量滴定板中孵育。孵育后，冲洗掉粘附细菌，并将剩余的粘附细菌（生物膜）用结晶紫染料染色，从而使生物膜可视化。如果需要定量，则将染色的生物膜溶解并转移至96孔光学透明平底板中，用于通过分光光度法进行测量。
The microtiter plate (also called 96-well plate) assay for studying biofilm formation is a method which allows for the observation of bacterial adherence to an abiotic surface. In this assay, bacteria are incubated in vinyl "U"-bottom or other types of 96-well microtiter plates. Following incubation, planktonic bacteria are rinsed away, and the remaining adherent bacteria (biofilms) are stained with crystal violet dye, thus allowing visualization of the biofilm. If quantitation is desired, the stained biofilms are solubilized and transferred to a 96-well optically clear flat-bottom plate for measurement by spectrophotometry.