Amplified fragment length polymorphism analyses of Eimeria spp.:: an improved process for genetic studies of recombinant parasites
Amplified fragment length polymorphism analyses of Eimeria spp.:: an improved process for genetic studies of recombinant parasites
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DOI：
10.1007/s00436-003-0890-x
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发表时间：
2003-08-01
影响因子：
2
通讯作者：
Shirley, MW
中科院分区：
文献类型：
--
作者：
Blake, DP;Smith, AL;Shirley, MW
扩增片段长度多态(AFLP)技术在遗传图谱研究中的应用需要高质量的DNA作为模板。在艾美耳球虫的例子中，这以前是从从大量卵囊中回收的纯化子孢子中获得的染色体DNA的形式(通常高达2x10(8))。为了使AFLP技术更容易地应用于极大艾美耳球虫卵囊数量较少的遗传学研究，建立了一种从少量卵囊中提取基因组DNA的简便、高效的方法。我们的新方法对其他球虫的遗传分析和从其他病原体中回收AFLP质量的DNA也是有用的。
Application of the amplified fragment length polymorphism (AFLP) technique to genetic mapping studies requires high quality DNA as a template. In the case of Eimeria spp., this has previously been in the form of chromosomal DNA obtained from purified sporozoites recovered from large numbers of oocysts (generally up to 2x10(8)). In order for the AFLP technique to be more easily applied to studies on the genetics of Eimeria maxima, for which only smaller numbers of oocysts are available, a simplified, more efficient method for the recovery of genomic DNA from small numbers of oocysts was developed. Our new method should also be useful for genetic analyses of other coccidial parasites and for the recovery of AFLP-quality DNA from other pathogens.