Peripheral Targets of 5-HT1D Receptor Immunoreactive Trigeminal Ganglion Neurons
Peripheral Targets of 5-HT1D Receptor Immunoreactive Trigeminal Ganglion Neurons
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DOI：
10.1111/j.1526-4610.2011.01862.x
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发表时间：
2011-05-01
期刊：
影响因子：
5
通讯作者：
Jennings, Ernest A.
中科院分区：
文献类型：
--
作者：
Ivanusic, Jason J.;Kwok, Matthew M. K.;Jennings, Ernest A.
客观。-本研究的目的是确定支配颅内血管和其他颅面组织的三叉神经初级传入神经元的比例，这些神经元也是5羟基曲丁胺（5-HT）（1D）受体免疫反应性的。逆行追踪和免疫组织化学用于鉴定5-HT 1D受体标记的三叉神经初级传入神经元，其支配泪腺（n = 3只动物）、鼻粘膜（n = 3只动物）和颅内脉管系统（硬脑膜中动脉[n = 3只动物]和大脑中动脉[n = 3只动物]）。支配脑膜中动脉的初级传入神经元的5-HT 1D受体免疫反应性神经元百分比（41.8 +/- 1%）高于支配大脑中动脉（28.4 +/- 0.8%）、鼻粘膜（25.6 +/- 1%）或泪腺（23.5 +/- 3%）的初级传入神经元。对于每个逆行标记群体，5-HT 1D受体免疫反应细胞是逆行标记细胞中最小的。这些发现为理解5-HT 1D受体激动剂（如曲普坦类）在治疗原发性血管性头痛中的作用提供了基础，并表明曲普坦类在治疗这些头痛中的选择性似乎并不是由5-HT 1D受体仅特异性定位于三叉神经血管神经元引起的。
Objective.-The aim of the current study was to determine the proportion of trigeminal primary afferent neurons that innervate the intracranial vasculature, and other craniofacial tissues, that are also 5 hydroxy triptamine (5-HT)(1D) receptor immunoreactive.Methods.-Retrograde tracing and immunohistochemistry was used to identify 5-HT1D receptor labeled trigeminal primary afferent neurons that innervate the lacrimal gland (n = 3 animals), nasal mucosa (n = 3 animals), and the intracranial vasculature (middle meningeal artery in the dura [n = 3 animals] and middle cerebral artery [n = 3 animals]).Results.-The percentage of neurons that were 5-HT1D receptor immunoreactive was greater for primary afferent neurons innervating the middle meningeal artery (41.8 +/- 1%) than those innervating the middle cerebral artery (28.4 +/- 0.8%), nasal mucosa (25.6 +/- 1%), or lacrimal gland (23.5 +/- 3%). For each retrograde labeled population, the 5-HT1D receptor immunoreactive cells were among the smallest of the retrograde labeled cells.Conclusions.-These findings provide a basis for understanding the role of 5-HT1D receptor agonists (eg, triptans) in the treatment of primary vascular headaches and suggest that the selectivity of triptans in the treatment of these headaches does not appear to result from specific localization of the 5-HT1D receptor to trigeminovascular neurons alone.